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Comprehensive Analysis of a lncRNA Based ceRNA Network and Prognostic Signature between Left- and Right-Side Colon Cancer
22 Pages Posted: 11 Jun 2019
More...Abstract
Background: Cancers located in the right- or left- side of the colon have distinct clinical and molecule characters. This study aimed to explore the regulatory mechanisms of location specific lncRNAs as ceRNA in colon cancer and identify potential prognostic biomarkers.
Method: Differentially expressed lncRNAs (DELs), miRNAs (DEMs) and mRNAs (DEGs) were screened between two side of colon cancers using RNA sequencing profiles, the functional enrichment analysis was implement for the DEGs, and ceRNA network was constructed. The association of DELs with patients' survival was analysis, and a DELs base signature was established to examine the prognostic value. Clinical tissues and GEO dataset were used to validate the results.
Results: 376 DELs, 35 DEMs and 805 DEGs were identified between two side of colon cancers. Functional enrichment analysis revealed the function and pathway of DEGs. A ceRNA network based on the 95 DELs -DEMs -DEGs interactions was constructed. Three DELs (LINC01555, AC015712, FZD10-AS1) were associated with overall survival of patients with colon cancer, a prognostic signature was established based on the three DELs, the results revealed that the high risk score of DELs signature indicated poor survival rates, and the signature has a good prognostic value on the colon cancers. Colon cancer tissues experiment and GEO dataset analysis verified the results.
Conclusion: The present study uncovered the role of location specific lncRNAs in colon cancer based on the ceRNA regulatory network, and identified a lncRNA based prognostic signature, which help to the prognosis and treatment in colon cancer patients.
Funding: This study was partially supported by research funding from the National Natural Science Foundation (No.81260083), Natural Science Foundation of Guangxi (No. 2018JJA140136), and Guangxi University Students Innovation and Entrepreneurship Project (WLXSZX19039; 201910598012).
Declaration of Interest: The authors declare that they have no conflict of interest.
Ethical Approval: This study was approved by the ethics committee of the Affiliated Tumor Hospital of Guangxi Medical University.
Keywords: colon cancer, location; lncRNA; ceRNA , prognostic signature
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