In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar Through Sucrose and Growth Regulators

Abstract

Microtubers which are produced in tissue culture have benefits for disease free potato production. As growth regulators and sucrose concentration affect the initiation and growth of microtuber, therefore, an experiment was conducted to discover the suitable growth supplement to produce disease free potato microtuber. Four potato cultivars viz. Granula, Daimond, Sarpoaxona and Lal Pakri1 with three different treatments viz. T1 = 8% Sucrose, T2 = 6% Sucrose + 4 mg/L Kinetin, T3 = 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP were used for micro tuberization as supplement of Murashige and Skoog (MS) medium. Among tested cultivars, Diamond performed better compare to other three potato cultivars with treatment combination T3. Maximum number of culture showing microtuber (56.0), explant formed microtuber (93.3%), number of microtuber (2.7), average diameter (4.42 mm) and average weight (97.61 mg) were found from Diamond cultivar in 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP.

Keywords: Potato cultivars, microtuberization, sucrose, kinetin, BAP