Download This Paper
Preprints with The Lancet is a collaboration between The Lancet Group of journals and SSRN to facilitate the open sharing of preprints for early engagement, community comment, and collaboration. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early-stage research papers that have not been peer-reviewed. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. The findings should not be used for clinical or public health decision-making or presented without highlighting these facts. For more information, please see the FAQs.
Clinically Accurate Diagnosis of Rheumatoid Arthritis and Systemic Lupus Erythematosusvia by Plasma Lipids Core Biomarkers
25 Pages Posted: 30 May 2020
More...Abstract
Background: To identify differential plasma lipidomics that could act as diagnostic biomarkers for rheumatoid arthritis(RA) and systemic lupus erythematosus(SLE) represents a current clinical challenge. Objective: We used large-scale lipidomics analysis as a novel strategy for investigating whether plasma lipidomics can identify useful biomarkers in the diagnosising of RA and SLE.
Methods: Here, we conducted a large-scale plasma lipidomics analysis by UPLC-MS/MS from 20 rheumatoid arthritis patients, 18 systemic lupus erythematosus patients and 20 healthy controls.
Results: We identified a sum of 467 lipids molecular features from each participant. Several lipids molecular provide high diagnostic value in the exact diagnosing of RA and SLE. Area Under Curve(AUC) were better than non-specific inflammation markers that found in blood ordinarily used to support in the diagnosis of RA and SLE respectively. MG(18:0/0:0/0:0) permitted to precise diagnosis of RA from healthy controls, with an AUC of 0.920, specificity 95% and sensitivity 80%. Proposed model of MG(16:0/0:0/ 0:0) yielded AUC 0.997, specificity 95% and sensitivity 100% in the diagnosis of SLE from healthy controls. Another PC183/181 by combination of PE160/204 and PE203/180 permitted to accurately diagnosis of RA from SLE, with 0.924 AUC, 70% specificity and 100% sensitivity.
Conclusion: Specific lipids changes in plasma are useful to precise diagnosis of RA or SLE from healthy controls, which would provide some clues for elucidating in the pathophysiology and potential therapeutic targets of these diseases.
Funding Statement: This work is funded by China National Natural Youth Science Foundation (81802078), Zhejiang Province Analysis Test Project Foundation (2018C37032), Zhejiang Province Public Welfare Research Foundation (GF20H200021), Zhejiang Provincial Department of Medicine and Health Foundation ( 2018KY912/2019RC315/2019323341/2020KY371), Enze Medical Center (Group) Scientific Research Foundation(18EZA06/15EZC2) and Luqiao Science and Technology Bureau Foundation (2017A23005).
Declaration of Interests: All authors declare no competing interests.
Ethics Approval Statement: The study protocol had been approved by Enze Group Ethics Committee and all of the participants had written consents for their blood sample to be used for research.
Keywords: rheumatoid arthritis, systemic lupus erythematosus, diagnosis, biomarkers, lipidomics
Suggested Citation: Suggested Citation