Download This Paper
Interaction of RSC Chromatin Remodelling Complex with Nucleosomes is Modulated by H3 K14 Acetylation and H2B SUMOylation In vivo
43 Pages Posted: 9 Apr 2020 Publication Status: Review Complete
More...Abstract
Chromatin remodelling complexes are multi-subunit nucleosome translocases that reorganize chromatin in the context of DNA replication, repair and transcription. A key question is how these complexes find their target sites on chromatin. Here, we use genetically encoded photo-crosslinker amino acids to map the footprint of Sth1, the catalytic subunit of the RSC (remodels the structure of chromatin) complex, on the nucleosome in living yeast. We find that the interaction of the Sth1 bromodomain with the H3 tail depends on K14 acetylation by Gcn5. This modification does not recruit RSC to chromatin but mediates its interaction with neighbouring nucleosomes. We observe a preference of RSC for H2B SUMOylated nucleosomes in vivo and show that this modification moderately enhances RSC binding to nucleosomes in vitro. Furthermore, RSC is not ejected from chromatin in mitosis, but its mode of nucleosome binding differs between interphase and mitosis. In sum, our in vivo analyses show that RSC recruitment to specific chromatin targets involves multiple histone modifications most likely in combination with other components such as histone variants and transcription factors.
Keywords: Genetic Code Expansion, Unnatural Amino Acids, Photo-crosslinking, Chromatin Remodelling, Lysine SUMOylation, Lysine acetylation
Suggested Citation: Suggested Citation