Optimization of Amylase Production From Nocardiopsis sp. DW-4 Isolated From Dwarka, Coastal Region of Gujarat

Kalasava, Ashish B.; Dangar, Kruti G.; Singh, Satya P.

doi:10.2139/ssrn.3571122

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Optimization of Amylase Production From Nocardiopsis sp. DW-4 Isolated From Dwarka, Coastal Region of Gujarat

Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS) 2020

10 Pages Posted: 8 Apr 2020 Last revised: 8 Jun 2020

See all articles by Ashish B. Kalasava

Kruti G. Dangar

Saurashtra University - Department of Biochemistry

Satya P. Singh

Saurashtra University

Date Written: April 6, 2020

Abstract

Halophilic actinomycete Nocardiopsis sp. DW-4 was isolated from Dwarka, the coastal region of Gujarat. The isolate was studied for the extracellular amylase production. The DW-4 strain displayed diversity with respect to growth patterns and production of extracellular amylase. ‘One factor variable at time’ method was used to obtain maximum amylase production. The influence of starch, salt and pH was studied on the amylase production and growth of DW-4. The isolate DW-4 could grow and produce amylase at 5-15% salt and starch 1-3% and produced optimum amylase at 5% NaCl and pH 8. Optimization of the amylase production in conjunction with the growth of the organisms is significant.

Keywords: Nocardiopsis sp., Amylase, Haloalkaliphilic actinomycetes

Suggested Citation: Suggested Citation

Kalasava, Ashish B. and Dangar, Kruti G. and Singh, Satya P., Optimization of Amylase Production From Nocardiopsis sp. DW-4 Isolated From Dwarka, Coastal Region of Gujarat (April 6, 2020). Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS) 2020, Available at SSRN: https://ssrn.com/abstract=3571122 or http://dx.doi.org/10.2139/ssrn.3571122