Download This PaperPreparation of Chlorpyrifos Degradation and Plant Growth Promoting Bacterial Formulation
14 Pages Posted: 10 Apr 2020 Last revised: 8 Jun 2020
Date Written: April 9, 2020
Abstract
Chlorpyrifos is an organophosphate pesticide used mainly in agriculture against the pests invading the crops. It becomes essential to degrade such pesticides that cause harm to the soil and the environmental biota. Pseudomonas putida is one of the nitrogen fixers found in the soil. In our study, we isolated and screened the chlorpyrifos degrading bacteria from pesticides contaminated agricultural soil. These bacterial isolates were identified and characterized by the VITEK® 2 Systems. The two isolates, namely J6 and J8, were identified based on their ability to grow at high concentrations of chlorpyrifos and used for further tests. The plant growth-promoting trains like production of Indole Acetic Acid, ammonia, protease, HCN, siderophore were analyzed. The phosphate solubilization ability and antimicrobial activities were also studied. A pot study of formulations prepared using J6 and J8 was conducted to examine the effects of these formulations on germination of seeds of Vigna radiata.
Keywords: Chlorpyrifos, Formulation, Pseudomonas putida, Plant growth promotion
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