The Study of the Prevalence of Different Microorganisms in Clinical Specimens at a Tertiary Care Hospital

7 Pages Posted: 13 Apr 2020 Last revised: 8 Jun 2020

See all articles by Dhaval Parmar

Dhaval Parmar

Indian Institute of Public Health Gandhinagar; M. V. M. Science and Home Science College

Nutan Prakash

Shree M. & N. Virani Science College

Valentina Umrania

M. V. M. Science and Home Science College

Nishith Vachhani

Life Blood centre

John J. Georrge

University of North Bengal

Date Written: April 10, 2020

Abstract

Bacterial infections are very common and diagnosis takes a minimum of three days. It is essential to treat patient empirically before report arrives as sometimes it may assert fatal consequences on the patient. 14891 clinical specimens of various infection sites were studied and cultured with the help of routine streak plate method or BacTec9050 machine, especially for blood and body fluids. If growth observed then identification was carried out using MicroScan autoScan4. 4990 specimens shown growth of either bacteria or Candida species. Stool culture shows highest positivity 84.2, followed by Pus 49.9%, Urine 46.3%, Tracheal aspirate 38.6%, Sputum 26.9%, Body fluids 9.2% and Blood shows lowest 4.8% positivity.64 different organisms were identified from this study. Gram-negative organisms dominated over Gram-positive and Candida species. E. coli was the most isolated organism with the share of 48.4% followed by K. pneumoniae (13.6%), P. aeruginosa (11.6%), A. baumannii (5.7%), S. aureus (5.0%) and C. albicans (2.0%). By assuming the causative agent before starting empirical treatment can act as lifesaving in critical situations. It also helps to choose the most suitable antibiotic from the pool instead of haphazard use.

Suggested Citation

Parmar, Dhaval and Prakash, Nutan and Umrania, Valentina and Vachhani, Nishith and Georrge, John J., The Study of the Prevalence of Different Microorganisms in Clinical Specimens at a Tertiary Care Hospital (April 10, 2020). Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS) 2020, Available at SSRN: https://ssrn.com/abstract=3573335 or http://dx.doi.org/10.2139/ssrn.3573335

Dhaval Parmar (Contact Author)

Indian Institute of Public Health Gandhinagar ( email )

Opp. Airforce Head quarter
Near Lekawada Bus Stop
Gandhinagar, 382042
India

M. V. M. Science and Home Science College ( email )

Rajkot
India

Nutan Prakash

Shree M. & N. Virani Science College ( email )

Department of Biotechnology
Shree M. & N. Virani Science College
Rajkot, Gujarat 360005
India

Valentina Umrania

M. V. M. Science and Home Science College

Microbiology Department
M. V. M. Science and Home Science College
Rajkot, Gujarat
India

Nishith Vachhani

Life Blood centre ( email )

Life Blood centre
Rajkot, Gujarat
India

John J. Georrge

University of North Bengal ( email )

Siliguri
Darjeeling Dist, West Bengal 734013
India
9898609230 (Phone)

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