The isocortex and hippocampal formation are two major structures in the mammalian brain that play critical roles in perception, cognition, emotion and learning. Using single-cell RNA-sequencing approaches, we profiled ~1.2 million cells covering all regions in the adult mouse isocortex and hippocampal formation. The cell types are organized hierarchically and exhibit varying degrees of discrete or continuous variations. Such molecular relationships correlate strongly with the spatial distribution patterns of the cell types, which can be region-specific, shared across multiple regions, or part of one or more gradients. Glutamatergic neuron types display much greater diversity than GABAergic neuron types, both molecularly and spatially, and define regional identities as well as inter-region relationships. Our study establishes a molecular architecture of the mammalian isocortex and hippocampal formation for the first time, and begins to shed light on its underlying relationship with the development, evolution, connectivity and function of these two brain structures.
Yao, Zizhen and Nguyen, Thuc Nghi and van Velthoven, Cindy T. J. and Goldy, Jeff and Sedeno-Cortes, Adriana E. and Baftizadeh, Fahimeh and Bertagnolli, Darren and Casper, Tamara and Crichton, Kirsten and Ding, Song-Lin and Fong, Olivia and Garren, Emma and Glandon, Alexandra and Gray, James and Graybuck, Lucas T. and Hawrylycz, Michael J. and Hirschstein, Daniel and Kroll, Matthew and Lathia, Kanan and Levi, Boaz and McMillen, Delissa and Mok, Stephanie and Pham, Thanh and Ren, Qingzhong and Rimorin, Christine and Shapovalova, Nadiya and Sulc, Josef and Sunkin, Susan M. and Tieu, Michael and Torkelson, Amy and Tung, Herman and Ward, Katelyn and Dee, Nick and Smith, Kimberly A. and Tasic, Bosiljka and Zeng, Hongkui, A Taxonomy of Transcriptomic Cell Types Across the Isocortex and Hippocampal Formation. Available at SSRN: https://ssrn.com/abstract=3575167 or http://dx.doi.org/10.2139/ssrn.3575167
This version of the paper has not been formally peer reviewed.
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