Download This Paper
Preprints with The Lancet is a collaboration between The Lancet Group of journals and SSRN to facilitate the open sharing of preprints for early engagement, community comment, and collaboration. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early-stage research papers that have not been peer-reviewed. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. The findings should not be used for clinical or public health decision-making or presented without highlighting these facts. For more information, please see the FAQs.
Inhibition Effect of Arresten Gene Transfection Combined with Nanoparticle-Mediated ENDOSTAR on Intimal Hyperplasia in Autogenous Vein Graft in Rats
21 Pages Posted: 13 Jul 2020
More...Abstract
Background: This study aims to investigate the inhibition effects of the Arresten gene with a nanoparticle-mediated target drug (Endostar, a recombinant human endostatin) on intimal hyperplasia in autogenous vein in rats.
Methods: Endostar nanoparticles and arresten gene nanoparticles were prepared in this study. The autogenous vein graft model was established through 80 Wistar rats. These rats were randomly divided into four groups (n = 20): group A (control group), group B (endostar nanoparticles group), group C (arresten gene nanoparticles group), and group D (arresten gene nanoparticles and endostar nanoparticles group). The vein grafts in each group were soaked in different treatments. The vein graft samples were harvested at four weeks post-operation. Then, intimal thickness was measured by computer image analysis after staining with hematoxylin-eosin. Immunohistochemical labeling and morphologic analysis of the vein graft sections were used to identify proliferating cell nuclear antigen positive cells.
Results: Intimal thickness in group D were significantly lesser than in group A (P < 0.01), as well as in groups B and C (both, P < 0.05). The number of proliferating cell nuclear antigen positive stained cells and the expression index of group D obviously decreased, compared with other three groups (P < 0.05).
Conclusions: Arresten gene nanoparticles loaded combined with Endostar nanoparticles are more effective than either drug alone.
Funding Statement: This study was funded by the Shandong Province Natural Science Foundation (No.ZR2009CM052) and Jinan City Bureau of Science and Technology Foundation (No.201401079).
Declaration of Interests: The authors declare that they have no competing interests.
Ethics Approval Statement: The animal protocol used was reviewed and approved by the Institutional Animal Care and Use Committee of the Shandong University. (No.ZR2009Cm052).
Keywords: arresten gene; endostar; intimal hyperplasia; autogenous vein graft; nanoparticle
Suggested Citation: Suggested Citation