Download This PaperSomatic Embryogenesis and Plant Regeneration From Stem, Leaf, Root and Petal Explants of Sweet Type Carambola (Averrhoea Carambola L.)
Sathyanarayana, B. N., Suma, G.B.S., Prashanta, K.G., Sondur, S. N. and Deepu Mathew, Somatic embryogenesis and plant regeneration from stem, leaf, root and petal explants of sweet type carambola (Averrhoea carambola L.). Journal of Plant Biology, 31 (2): 131-137, 2004
DOI: 10.5281/zenodo.836502
7 Pages Posted: 10 Jun 2020
Date Written: June 10, 2004
Abstract
The effect of different plant growth regulator concentrations and combinations in direct and indirect somatic embryo-genesis on Averrhoa carambola explants of in vitro raised immature and mature in situ origin was studied. Percent response to callus induction from leaf explants of both origin was best on half strength MS medium supplemented with 2 mg/L 2,4-D. Maximum direct shoot regeneration from leaf explants of in vitro origin was on medium with 6 mg/L NAA. NAA at 8 mg/L and 2,4-D at 6 mg/L were equally good for inducing callus on petal explants. Basal stem and root explants of in vitro origin cultured on half strength MS medium supplemented with 2 and 4 mg/L BAP produced maximum number of somatic embryos directly.
Keywords: In vitro, Averrhoa, Plant Tissue Culture, Micro-propagation
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