Download This PaperIn Vitro Callus Induction and Plant Regeneration in Roseapple (Syzygium Jambos L.)
Prashantha, K. G., Sathyanarayana, B. N., Deepu Mathew and Sondur, S. N., 2003, In vitro callus induction and plant regeneration in roseapple (Syzygium jambos L.). Journal of Plant Biology, 30 (1): 99-102. doi: 10.5281/zenodo.836504
4 Pages Posted: 11 Jun 2020
Date Written: October 12, 2003
Abstract
In vitro callus induction from the cotyledon and shoot morphogenesis from nodal explants were obtained in rose-apple. Freshly extracted seeds were surface sterilized best with 80 per cent ethyl alcohol for 10 s followed by 2.5 per cent sodium hypochlorite for 10 min. When inorganic salt concentration for MS medium was reduced to quarter strength, the browning was reduced and survival per cent was high. Both 2,4-D and NAA at 2 mg/L induced light green compact callus on cotyledonary explants. Highest number of shoots (4.1) with maximum leaves (5.0) were proliferated from nodal explants on MS medium with BAP at 4 mg/L. Rooting of the induced shoots was obtained on medium with 6 mg/L IBA. In vitro morphogenesis strategies in rose-apple along with parallel reports are discussed.
Keywords: syzygium jambos, rose-apple, in vitro, micropropagation, fruit, horticulture
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