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Adherens Junction Engagement Regulates Functional Patterning of the Cardiac Pacemaker Cell Lineage
50 Pages Posted: 13 May 2020 Publication Status: Published
More...Abstract
Cardiac Pacemaker Cells (CPCs) rhythmically initiate the electrical impulses that drive heart contraction. CPCs display the highest rate of spontaneous depolarization in the heart despite being subjected to inhibitory electrochemical conditions that should theoretically suppress their activity. While several models have been proposed to explain this apparent paradox, the actual molecular mechanisms that allow CPCs to overcome electronic barriers to their function remain largely unexplored. Here we have traced CPC development at single cell resolution and uncovered a series of cytoarchitectural patterning events that are critical for proper pacemaking. Specifically, our data reveal that CPCs dynamically modulate adherens junction (AJ) engagement to control characteristics including surface area, volume, and gap junctional coupling. This allows CPCs to adopt a structural configuration that supports their overall excitability. Thus, our data have identified a previously unrecognized role for local cellular mechanics in patterning critical morphological features that are necessary for CPC electrical activity.
Keywords: Sinoatrial Node, Cardiac Pacemaker Cell, Adherens Junction, Electrophysiology, Gap Junction, Cytoarchitecture, Cardiac Development
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