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The Novel Antimicrobial Activities of Carrimycin Against Multidrug Resistant Acinetobacter baumannii and Pseudomonas aeruginosa Systemic Infection
40 Pages Posted: 10 Aug 2020
More...Abstract
Background: The multidrug-resistant(MDR) Acinetobacter baumannii and Pseudomonas aeruginosa have been listed as the top two critical pathogens to threat the public health by the World Health Organization (WHO) and regarded as a global priority for investment in new drugs. Carrimycin is a novel macrolide antibiotic used in therapy of community acquired respiratory infection. In this study, we want to further examine its antibacterial effect against the MDR A. baumannii and P. aeruginosa in vitro and in vivo.
Methods: In vitro, we tested the minimal inhibitory concentration(MIC) of the strains of A. baumannii or P. aeruginosa isolated from clinic or ATCC. In vivo, using the wild-type(WT) Balb/c mice and T-cell-deficient Balb/c nude mice bacteremia model, we examined the count of A. baumannii and P. aeruginosa colony culture and histopathological damage in target organs tissue after carrimycin administration.
Findings: Our results demonstrated that most strains of A. baumannii and P. aeruginosa were resistant to drugs and inhibited growth by carrimycin partly in vitro, but could be cleaned in infected Balb/c mice with the dose-dependent manner, but not in Balb/c nude mice. Inflammation relief in target organs also be observed in Balb/c mice but not in Balb/c nude mice.
Interpretation: These findings indicated that carrimycin could not only clean pathogens directly, but also could improve the host's immunity to anti-inflammation and relieve damage against A. baumannii and P. aeruginosa infection.
Funding: This work was funded by the National Emergency Response Program of China (Grant number 2020YFC0844900) and the National Natural Science Foundation of China (Grant number 81872852).
Declaration of Interests: The authors declare no conflicts of interest.
Ethics Approval Statement: The experimental procedures were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the local Animal Care and Use Committee.
Keywords: carrimycin, antimicrobial activities, multidrug resistant, Acinetobacter baumannii, Pseudomonas aeruginosa, infection
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