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Unveiling Molecular Recognition of Sialoglycans by Human Siglec-10
31 Pages Posted: 13 May 2020 Publication Status: Published
More...Abstract
Siglec-10 is an inhibitory I-type lectin, selectively recognizing sialoglycans exposed on cell surfaces, involved in several patho-physiological processes. The key role Siglec-10 plays in the regulation of immune cell functions has made it a potential target for the development of immunotherapeutics against a broad range of diseases. Though, a detailed understanding of sialoglycans recognition by Siglec-10 is still an unmet challenge; neither the crystal structure of the protein nor the atomic description of its interactions with complex glycans had been indeed previously described.We present here the first insights of the molecular mechanisms regulating the interaction between Siglec-10 and naturally occurring sialoglycans. We used combined spectroscopic, computational and biophysical approaches to dissect glycans’ interactome and conformation upon binding, and to afford a description of the 3D complexes. Our outcomes provide a privileged structural perspective for the rational design and development of novel high affinity ligands to control the receptor functionality.
Keywords: Siglecs, molecular recognition, N-glycans, NMR
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