puc-header

Mask, a Component of the Hippo Pathway, is Required For  Drosophila Eye Morphogenesis

69 Pages Posted: 25 Oct 2019 Publication Status: Review Complete

See all articles by Miles W. DeAngelis

Miles W. DeAngelis

Wesleyan University - Department of Biology

Emily W. McGhie

Wesleyan University - Department of Biology

Joseph D. Coolon

Wesleyan University - Department of Biology

Ruth I. Johnson

Wesleyan University - Department of Biology

More...

Abstract

Hippo Signaling is a regulator of tissue growth, but we show that the Yki and its cofactor Maskalso have a critical role in the morphogenesis of the post-mitotic Drosophila pupal eye. Mask is required for the correct distribution and accumulation of adherens junctions and appropriate organization of the cytoskeleton. Accordingly, disrupting mask expression led to severe patterning defects. Similar phenotypes were observed when yki was reduced or in response to ectopic wts and mis-patterning phenotypes observed when mask expression was reduced were modified by Hippo pathway activity, supporting our hypothesis that Mask’s function in fly eye patterning is within the context of Hippo signaling. RNA-sequencing analyses revealed that Mask regulates the transcription of numerous genes associated with cytoskeletal organization and cell adhesion, including FER kinase that phosphorylates β-Catenin and Vinculin. Similar trends in FER and Vincexpression were observed when yki was reduced, suggesting that Mask:Yki complexes contribute to the adhesive properties and architecture of retinal cells. Since it is well-established that Hippo pathway activity is responsive to changes in cell adhesion and the cytoskeleton, our data support that a feedback loop utilizes Hippo signaling to both sense and regulate adhesion and the cytoskeleton to fine-tune morphogenesis.

Keywords: Morphogenesis, Drosophila, eye, Hippo, Yorkie, Mask

Suggested Citation

DeAngelis, Miles W. and McGhie, Emily W. and Coolon, Joseph D. and Johnson, Ruth I., Mask, a Component of the Hippo Pathway, is Required For  Drosophila Eye Morphogenesis (October 23, 2019). Available at SSRN: https://ssrn.com/abstract=3474683 or http://dx.doi.org/10.2139/ssrn.3474683
This version of the paper has not been formally peer reviewed.

Miles W. DeAngelis

Wesleyan University - Department of Biology ( email )

United States

Emily W. McGhie

Wesleyan University - Department of Biology ( email )

United States

Joseph D. Coolon

Wesleyan University - Department of Biology ( email )

United States

Ruth I. Johnson (Contact Author)

Wesleyan University - Department of Biology ( email )

United States

Click here to go to Cell.com

Paper statistics

Downloads
23
Abstract Views
441
PlumX Metrics