Impact of Swcnts and Cmcs on Efficacy of Anastomosis and Optical Properties in Vivo During Laser Biological Tissue Soldering

Abstract

Laser assisted anastomosis has become the focus of surgical operation research due to its obvious advantages, but the improvement of capability of incision anastomosis has entered the bottleneck period. In order to improve the anastomosis quality of skin tissue incision, further shorten the healing cycle and the tendency of skin fibrosis, the effects of CMCS or SWCNT combined with 25%BSA and 0.4%ICG on laser tissue soldering were investigated in this paper. In this study, Nd:YAG pulsed laser was used to perform wound anastomosis tests on living rats. The skin wound was scanned by 67.3J/cm2 laser along the incision. The wound anastomosis performances of different types and concentrations of biological dressings applied at different locations were experimented. The results showed that the biocompatible dressing containing CMCS and SWCNT had a significant effect on the promoting wound anastomosis after laser welding. Tensile strength of the dressing group without CMCS or SWCNT after laser welding is between 6.5-9.8N/cm2, while that of the dressing group with CMCS is 26.5N/cm2. Macro morphology of wound healing showed that the healing period was significantly shortened by CMCS and SWCNT dressing, healing cycle reduced from 14 days to 9-10 days. The optical properties of skin tissue under different dressing were tested. It was proved that 5%CMCS and 4%SWCNT could change the laser absorption rate of skin tissue, improve the photothermal conversion efficiency, and significantly improve the instantaneous anastomosing strength of wound. Ceravine scarlet staining and Masson staining showed that carbon nanoparticles promoted collagen skeleton regeneration when the dressing of 0.4%SWCNT was applied internally, and the tendency of fibrosis was increased after 14 days of laser welding. The combination of CMSC and SWCNT may further improve the incision anastomosis strength and control laser heat damage in the future.

Note:
Funding Information: Qinglan Project of Jiangsu Province -AD41069

Declaration of Interests: No competing interest need to be declared.

Ethics Approval Statement: All animal experiments were complied with the ARRIVE guidelines and were carried out in accordance with the U.K. Animals (Scientific Procedures) Act 1986 and associated guidelines. Our experiments were approved by our ethics committee, our Experimental ethics number (IACUC): IACUC2103014; Feeding contract number: SYHT20210064.

Keywords: Laser welding, Skin tissue, CMCS, SWCNT, Biological dressing, Wound anastomosis