26S rRNA Gene Is not Useful for Identifying Saccharomycodes Ludwigii by qPCR

2 Pages Posted: 14 Feb 2022

See all articles by Wenfa Ng

Wenfa Ng

National University of Singapore (NUS)

Date Written: November 26, 2021

Abstract

Through intensive research and development, quantitative polymerase chain reaction (qPCR) has emerged as a dominant tool for precision clinical detection of many pathogenic microbial species in difficult-to-analyse sample matrixes. The method requires precise and specific targeting of unique species-identifying regions of particular gene in a microbe. To do this, species-specific genes need to be identified, and this is followed by identification of short stretches of DNA sequence that could uniquely identify the microbe from amongst billions of possible candidates. Multiple sequence alignment of 26S rRNA gene of 7 different strains of Saccharomycodes ludwigii was conducted in this study to help identify short (~100 bp) regions in the gene that could uniquely identify the microbe in qPCR assays. Results revealed that no such short DNA segments that could be used to identify S. ludwigii in qPCR assays. Hence, 26S rRNA gene is not a good candidate for designing primers that could uniquely identify S. ludwigii.

Keywords: Saccharomycodes ludwigii, quantitative polymerase chain reaction, 26S rRNA gene, microbial identification

Suggested Citation

Ng, Wenfa, 26S rRNA Gene Is not Useful for Identifying Saccharomycodes Ludwigii by qPCR (November 26, 2021). Available at SSRN: https://ssrn.com/abstract=3972081 or http://dx.doi.org/10.2139/ssrn.3972081

Wenfa Ng (Contact Author)

National University of Singapore (NUS) ( email )

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