A Turn-On Aie Dual Channel Fluorescent Probe for Sensing Cr3+/Clo- and Application in Cell Imaging

Abstract

Using dansyl hydrazide and 4, 6-dimethoxysalicylaldehyde as raw materials, a Cr3+/ClO- enhanced fluorescent probe DNS (5-(dimethylamino)-N'-(2-hydroxy-4, 6-dimethoxyenzylidene)-naphthalene-1-sulfonyl hydrazide) with AIE effect was synthesized. Probe DNS can quickly and high selectively recognize Cr3+/ClO- in solvent system (H2O/DMSO=2:8). The binding of probe DNS with Cr3+/ClO- produces significant fluorescence enhancement effect, which is almost unaffected by other ions. After calculation, the detection limits (LOD) of probe DNS are 5.3588×10-7 mol/L(Cr3+) and 3.6548×10-7 mol/L (ClO-) respectively. The binding mechanism of probe DNS with Cr3+/ClO- was explored using Job's curve, 1HNMR titration, and mass spectrometry. In addition, the low cytotoxicity and biocompatibility of probe DNS provide potential for testing exogenous Cr3+/ClO- and endogenous ClO- in living cells. Probe DNS is promising for real-time detection and bioimaging.

Keywords: Naphthyl hydrazide, AIE effect, Fluorescent probe, Mechanism, bioimaging