Bio-Control Ability of Lactic Acid Bacteria and Bifidobacteria Against Ochratoxin a During Grape Juice Fermentation
32 Pages Posted: 7 May 2022
Abstract
Ochratoxin A (OTA) is a secondary metabolite produced by Aspergillus and Penicillium, and poses a serious threat to food safety. The control of OTA has always been an important aspect in food processing. In this study, the removal ability of lactic acid bacteria (LAB) and bifidobacteria against OTA during grape juice fermentation was investigated. Firstly, the fermentation performance of 12 strains was measured and 11 of them with good fermentation ability were obtained. Then, principal component analysis was used to comprehensively score the ability of the bacteria to remove OTA under different initial OTA concentrations and fermentation times, and 4 strains with the highest score were obtained. After that, Lacticaseibacillus rhamnosus 6133 (Lr-6133) was screened out through OTA tolerance test and ultimate tolerance test. Finally, the Box-Behnken Design in Response Surface Methodology was used to optimize the culture conditions of Lr-6133. With the inoculation amount of 7%, fermentation temperature of 35°C and fermentation time of 24 h, the maximum OTA removal rate was 35.34%. These results indicated that Lr-6133 could not only accords with the physicochemical indexes of fermented grape juice, but also had the best effect of comprehensive removal of OTA in grape juice.
Keywords: lactic acid bacteria, Ochratoxin A, Grape juice, fermentation, Detoxification
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