Determination of S-100b Protein Immunoreactivity in Patients with Acute Ischemic Stroke
The International Conference Education and Creativity for a Knowledge Based Society – Medicine and Dental Medicine, 2012
Posted: 19 Mar 2013
Date Written: 2012
Background: Dysfunction and damage of the human central nervous system can be detected with S-100B protein.
Objective: To assess S-100B protein immunoreactivity in blood samples from patients with acute ischemic stroke and its correlation with clinical outcome. Materials and Methods Serum concentrations of S-100B protein immediately after admission for acute ischemic stroke in 67 patients (38 female, 29 male patients, age 63-85 years, mean age 68.4 years), compared to 66 control subjects were measured. The concentration of S-100B protein in blood samples was measured in duplicate using enzyme linked immunosorbent assay (cut off of 8 pg/mL).
Significance: Differences between groups was calculated by the Wilcoxon signed rank method. A value of p<0.05 was considered significant. Results The mean value concentration of S-100B was significantly higher than mean value found in the control group (395 pg/mL±150 pg/mL vs. 109 pg/mL±44.5 pg/mL, p=0.0001). The serum concentrations of S-100B protein ranged from 95 pg/mL to 2016 pg/mL. Concentrations in the control group ranged from 20 pg/mL to 657 pg/mL. This marker correlated significantly with clinical outcome (p<0.001). Elevated levels are not diagnostic of acute ischemic stroke, the negative predictive value of an undetectable S-100B protein level was 0.92. Determination of S-100B protein in serum may be used to select patients for CT scanning. The results confirm that inflammatory proteins are released into the blood after stroke, they can be easily measured, and they correlate with clinical outcome.
Conclusion: Higher peak plasma levels of S-100B protein plasma levels correlated well with worse clinical outcome. This biochemical marker is not specific but indicates any cell damage in the central nervous system.
Keywords: S-100B protein, acute ischemic stroke, clinical outcome
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