12 Pages Posted: 10 May 2013 Last revised: 5 Jan 2016
Date Written: May 7, 2013
In this study, the levels of genetic variability were estimated in S.richadsonii fish populations from three different locations viz., Chirapani stream (pop 1), Gola (pop 2) and KosiRiver (pop 3) by using OPY Operon primers and 10 primers were produced distinct and repeatable amplification as well polymorphic loci. The higher proportion of polymorphic loci was observed in Champawat population as compared to other two populations and with the overall % polymorphisms were 14.76. The OPY 01, OPY 04, OPY 11 and OPY 16 primers were found to be the population specific banding patterns among these three populations. The Nei's genetic diversity (h) was found to be highest in pop1 andpop2 with 0.24 and 0.2 in pop3. The total gene diversity (Ht) in the population was 0.2962, within sample gene diversity (Hs) of 0.2321 and the genetic differentiation (GST) among the populations was 0.2164. The largest genetic distance (0.1565) was obtained between pop1 and pop2, whereas the smallest genetic distance was between pop1 and pop3 (0.1058), followed by pop3 and pop2 (0.1385). Further it is suggested that the levels of genetic identity and diversity obtained was very low and might be due to the less number of polymorphic loci and migration rate.
Keywords: Coldwater Fish, RAPD, Genetic Diversity, Markers, Population
Suggested Citation: Suggested Citation
Sivaraman, Gopal Krishnan and Barat, A. and Ali, S. and Joshi, K.D. and Mahanta, P. C., Assessment Population Genetic Structure and Diversity among Indian Snow Trout (S.richardsonii) Fish Species (May 7, 2013). Available at SSRN: https://ssrn.com/abstract=2261713 or http://dx.doi.org/10.2139/ssrn.2261713