Organogenesis of Ammi Majus Via Callus Culture from Leaf

Plant Tissue Culture and Biotechnology, 23(1), 101-106.

6 Pages Posted: 6 Aug 2013

See all articles by Sandip Magdum

Sandip Magdum

R.D. Aga Research, Technology and Innovation Centre, Thermax Ltd.

Sanjeev Kumar

Amity University - Amity Institute of Biotechnology

Date Written: July 2, 2013

Abstract

Ammi majus L. is an imperiled medicinal plant, which contains various medically important and uncommon extraordinary secondary metabolite compounds.. An efficient organogenesis protocol was established for Ammi majus through in vitro process of leaf callusing, shooting and rooting by using different combination of auxins and cytokinins. In vitro cultured juvenile leaf explants were used directly for callus formation on MS supplemented with different concentration of IAA, Kn and CH. Maximum percentage response for callus formation was 97.7 after 5 weeks of incubation on MS supplemented with 2 mg/l IAA, 2 mg/l Kn and 1000 mg/l CH. About 81% of calluses converted to shoot on callusing medium supplemented with 50 mg/l glutamine and 40 mg/l adenine, after 80 days of culture. Plantlets with shoot were transferred to half strength of MS with different concentration of IBA and glutamine. Maximum rooting of 75.96% was observed on half strength of MS supplemented with 0.2% of IBA and 100 mg/l glutamine. The present study gives reliable in vitro regeneration protocol for Ammi majus L. from leaf explants, which may be used for future commercial‐scale propagation and genetic study.

Keywords: Ammi majus, organogenesis, regeneration

Suggested Citation

Magdum, Sandip and Kumar, Sanjeev, Organogenesis of Ammi Majus Via Callus Culture from Leaf (July 2, 2013). Plant Tissue Culture and Biotechnology, 23(1), 101-106.. Available at SSRN: https://ssrn.com/abstract=2306502

Sandip Magdum

R.D. Aga Research, Technology and Innovation Centre, Thermax Ltd. ( email )

Pune, 411019
India

Sanjeev Kumar (Contact Author)

Amity University - Amity Institute of Biotechnology ( email )

J-3 Block, Amity University Campus
Sector – 125
Noida
India

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