Organogenesis of Ammi Majus Via Callus Culture from Leaf
Plant Tissue Culture and Biotechnology, 23(1), 101-106.
6 Pages Posted: 6 Aug 2013
Date Written: July 2, 2013
Ammi majus L. is an imperiled medicinal plant, which contains various medically important and uncommon extraordinary secondary metabolite compounds.. An efficient organogenesis protocol was established for Ammi majus through in vitro process of leaf callusing, shooting and rooting by using different combination of auxins and cytokinins. In vitro cultured juvenile leaf explants were used directly for callus formation on MS supplemented with different concentration of IAA, Kn and CH. Maximum percentage response for callus formation was 97.7 after 5 weeks of incubation on MS supplemented with 2 mg/l IAA, 2 mg/l Kn and 1000 mg/l CH. About 81% of calluses converted to shoot on callusing medium supplemented with 50 mg/l glutamine and 40 mg/l adenine, after 80 days of culture. Plantlets with shoot were transferred to half strength of MS with different concentration of IBA and glutamine. Maximum rooting of 75.96% was observed on half strength of MS supplemented with 0.2% of IBA and 100 mg/l glutamine. The present study gives reliable in vitro regeneration protocol for Ammi majus L. from leaf explants, which may be used for future commercial‐scale propagation and genetic study.
Keywords: Ammi majus, organogenesis, regeneration
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