Analytical Methods for the Selected Properties Determination of 3-hydroxypropionaldehyde (HPA)
Chuen-Tat Kang (2014). Analytical methods for the selected properties determination of 3-hydroxypropionaldehyde (HPA). Sky Journal of Biochemistry Research, 3(1) : 42 – 46
9 Pages Posted: 10 Jul 2017
Date Written: January 1, 2014
3-hydroxypropionaldehyde (3-HPA) is an intermediate metabolite of glycerol metabolism towards the formation of 1,3-propanediol. Voisenet (1910) in fact has observed this substance and its formation from glycerol during bacterial spoilage of wine. Being one of the antibiotics and precursors for high quality plastics, unfortunately there are still no complete set of analytical data available for this substance. From the experimental data from this paper, NMR analysis using 2-H showed that –CHO bond of aldehyde observed at δ9.5, RCH-3 at δ1.37, R-2 CH-2 at δ3.68, R-3 CH at δ1.9 and so on. The carbon-13 NMR spectrum showed six signals, at chemical shifts of 40.1, 46.3, 56.2, 58.7, 89.7, and 207.7 ppm. In FTIR, spectral contained a broad O-H stretch band at 3,450 cm^(-1) and a large peak due to C-O stretching at 1,050 to 1,150 cm^(-1), indicating hydroxyl functional group. A C-H stretches at 2,880 and 1,380 along with a C=O stretch typical of aldehydes was present at 1,730 cm^(-1). All the analytical information above is similar to the previous record (Talarico, 1989). GPC data, although not fully accurate, will help to estimate the molecular weight of 3-HPA as 257 with 63% errors. The HPLC data with three peaks may reveal the three components of 3-HPA available. SEM analysis did not reveal any important solid data but revealing its solid state as homogenous particle. By applying Circle plot, quantitative data on the concentration of 3-HPA synthesized chemically is estimated to be 19.62 μmol / ml at absorbance of 600 nm.
Keywords: 3-hydroxypropionaldehyde, NMR, acrolein, propanediol, glycerol fermentation
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