Utilization of Thiocyanate (SCN-) by a Metabolically Active Bacterial Consortium as the Sole Source of Cellular Nitrogen.
Patil Yogesh B (2011) Utilization of thiocyanate (SCN-) by a metabolically active bacterial consortium as the sole source of nitrogen. International Journal of Chemical, Environmental & Pharmaceutical Research, 2: 44-48 (ISSN: 2229-3892-Print, 2229-5283-Online)
5 Pages Posted: 16 Nov 2014
Date Written: November 15, 2014
Thiocyanate (SCN-), a toxic chemical species from cyanide family, consists of both carbon (C) and nitrogen (N) in equimolar ratio and is being emanated through liquid effluents by several industrial processes. Since bioremediation technologies for waste treatment are gaining enormous importance in the recent times; microbial treatment of SCN- is therefore being researched worldwide. However, utilisation of SCN- by microbes as a suitable growth substrate (C and/or N source) is poorly understood and presents the problem in waste treatment systems. A heterotrophic bacterial consortium comprising of three Pseudomonas sp. isolated from activated sludge and having potentials for environmental clean-up, was capable of utilising thiocyanate (SCN-) from aqueous solutions as the sole source of cellular nitrogen (N) in the presence of carbon (C) source viz. glucose. The consortium ceased to grow and degrade SCN- when supplemented with C and N sources alone. Diauxie pattern was observed when the consortium culture was supplied with two N sources (NH4Cl and SCN-) in the presence of glucose as a source of carbon (C). NH4Cl was the preferred growth substrate utilised by the bacterial consortium followed by SCN-.
Keywords: Biodegradation, Bacterial consortium, Diauxie, Nitrogen source, Thiocyanate.
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