Development of a Novel Inoculum Preparation Method for Solid-State Fermentation -- Cellophane Film Culture (CFC) Technique

Industrial Crops and Products, 43, 774-777.

13 Pages Posted: 19 Jan 2015

See all articles by Teck Nam

Teck Nam

The University of Auckland

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Adeline Seak May Chua

University of Malaya (UM)

Date Written: 2013

Abstract

This study reports a user-friendly technique in the preparation of fungal inoculum intended for solid-state fermentation (SSF) – Cellophane Film Culture (CFC) technique. This technique uses cellophane film-overlaid agar plates to facilitate the separation of fungal biomass. The findings showed that inoculum of P. sajor-caju produced is viable, and it was confirmed by the presence of laccase enzyme activity in SSF of rice husk. The correlation between fungal dry and wet weights (r2 = 0.9329) provides an accurate estimation of fungal dry weight from its wet weight during inoculum preparation. Besides, this technique does not require a strict sterile handling condition and possesses lower risk of contamination compared with liquid culture and agar plugs approaches. In consideration of large scale inoculum preparation, this newly developed technique is comparatively more cost-effective, which further suggests its potential in inoculum preparation from mycelial fungi for SSF.

Keywords: Inoculum; solid-state fermentation; cellophane; fungus; rice husk

JEL Classification: C09, C93, N55, O13, Q00, Q10, Q16, Q20, Q23, Q40, Q42

Suggested Citation

Ang, Teck Nam and Ngoh, Gek-Cheng and Chua, Adeline Seak May, Development of a Novel Inoculum Preparation Method for Solid-State Fermentation -- Cellophane Film Culture (CFC) Technique (2013). Industrial Crops and Products, 43, 774-777.. Available at SSRN: https://ssrn.com/abstract=2550035

Teck Nam Ang (Contact Author)

The University of Auckland ( email )

Department of Chemical and Materials Engineering
Auckland, AK 1010
New Zealand

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Adeline Seak May Chua

University of Malaya (UM) ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
University of Malaya (UM)
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

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