Evaluation and Degradation Chemistry of Orbifloxacin Using LC-MS

International Journal of Sciences 2013 (09)

10 Pages Posted: 10 Jul 2017

See all articles by Edith Laignier Cazede

Edith Laignier Cazede

Universidade Estadual Paulista - School of Pharmaceutical Sciences

Sanjay Garg

University of South Australia - School of Pharmacy and Medical Sciences

Herida Nunes Salgado

Universidade Estadual Paulista - School of Pharmaceutical Sciences

Date Written: September 2, 2013

Abstract

Orbifloxacin (ORBI) was subjected to different ICH (Q1A(R2)) prescribed stress conditions of thermal stress, hydrolysis, oxidation and photolysis. It was stable to dry heat (60 ºC) and photolysis (UV-VIS) in solid form. It showed extensive decomposition under hydrolytic and photolytic conditions in acid, base and neutral solutions. Degradation was also observed in oxidative condition. Degradation products of ORBI formed under different forced conditions were characterized through LC–MS studies. In total, eleven major degradation products (DP1-DP11) were detected. Successful separation of drug and degradation products formed under various stress conditions was achieved on a analytical column SymmetryShield Waters RP18, 5 µm, 250 x 4.6 mm using 5% acetic acid and methanol as mobile phase in a gradient mode at flow rate of 0.5 ml/min. The peaks were detected using a PDA detector set at 290 nm. The method was extended to LC–APCI-MS for characterization of the degradation products and the pathways of decomposition were proposed.

Keywords: Orbifloxacin, Chemistry degradation, SIAM, LC-APCI-MS, Stability

Suggested Citation

Laignier Cazede, Edith and Garg, Sanjay and Nunes Salgado, Herida, Evaluation and Degradation Chemistry of Orbifloxacin Using LC-MS (September 2, 2013). International Journal of Sciences 2013 (09). Available at SSRN: https://ssrn.com/abstract=2572795

Edith Laignier Cazede (Contact Author)

Universidade Estadual Paulista - School of Pharmaceutical Sciences ( email )

Araraquara SP
Brazil

Sanjay Garg

University of South Australia - School of Pharmacy and Medical Sciences

37-44 North Terrace, City West Campus
Adelaide, 5001
United States

Herida Nunes Salgado

Universidade Estadual Paulista - School of Pharmaceutical Sciences

Araraquara SP
Brazil

Here is the Coronavirus
related research on SSRN

Paper statistics

Downloads
9
Abstract Views
127
PlumX Metrics