Antimicrobial Activity, Toxicity and Stability of Phytol as a Novel Surface Disinfectant

Environmental Health Engineering and Management Journal 2015; 2(1): 13–16

4 Pages Posted: 22 Jun 2015

See all articles by Mohammad Taghi Ghaneian

Mohammad Taghi Ghaneian

Environmental Health Engineering Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Mohammad Hassan Ehrampoush

Department of Environmental Health Engineering, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Ali Jebali

Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Seyedhossein Hekmatimoghaddam

Shahid Sadoughi University of Medical Sciences, School of Paramedicine, Department of Laboratory Sciences

Mohammad Mahmoudi

Department of Environmental Health, Faculty of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Date Written: January 20, 2015

Abstract

Background: Although various surface disinfectants have been introduced, most of them are toxic. The use of natural antimicrobial agent e.g. phytol, extracted from Leptadenia pyrotechnicais a new strategy. The aim of this study was to evaluate the antimicrobial activity, toxicity, and stability of phytol.

Methods: The serial concentrations of phytol were prepared, and separately incubated with four microbial isolates. Then, its Minimum Inhibitory Concentration (MIC) was measured for each microorganism. For toxicity test, serial concentrations (62.5, 125, 250, 500 and 1000 μg/mL) of phytol were incubated with mouse skin cells, and then cell viability was calculated by MTT assay. For stability test, three common surfaces (stone, steel, and MDF) were considered. Then, 100 μL of phytol was separately spread over their surface, and they have been kept at lab panel for 12, 24 and 36 hours. After incubation, two samples were obtained from each surface and inoculated on nutrient agar plates. Finally, colony count was read for each surface. T-test was used to evaluate the significant differences between groups, and P>0.05 considered as level of significant difference.

Results: The MIC50 of phytol against E.coli, C.albicans, and A.nigerwas 62.5 μg/mL, and against S.aureuswas >1000 μg/mL. MTT assay showed that the toxicity of phytol was dose and time dependent. The stability test demonstrated that phytol was stable on the stone, MDF, and steel surfaces until 36 hours.

Conclusion: It can be concluded that phytol has high antimicrobial activity, high stability, and low toxicity. This substance must be evaluated at actual conditions.

Keywords: Antimicrobial activity, Phytol, Toxicity, Stability

Suggested Citation

Ghaneian, Mohammad Taghi and Ehrampoush, Mohammad Hassan and Jebali, Ali and Hekmatimoghaddam, Seyedhossein and Mahmoudi, Mohammad, Antimicrobial Activity, Toxicity and Stability of Phytol as a Novel Surface Disinfectant (January 20, 2015). Environmental Health Engineering and Management Journal 2015; 2(1): 13–16, Available at SSRN: https://ssrn.com/abstract=2610015

Mohammad Taghi Ghaneian

Environmental Health Engineering Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ( email )

Shohaday-e-Gomnam Blvd.
Alem Sq.
Yazd
Iran

Mohammad Hassan Ehrampoush

Department of Environmental Health Engineering, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ( email )

Shohaday-e-Gomnam Blvd.
Alem Sq.
Yazd
Iran

Ali Jebali

Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ( email )

Shohaday-e-Gomnam Blvd.
Alem Sq.
Yazd
Iran

Seyedhossein Hekmatimoghaddam

Shahid Sadoughi University of Medical Sciences, School of Paramedicine, Department of Laboratory Sciences ( email )

Iran

Mohammad Mahmoudi (Contact Author)

Department of Environmental Health, Faculty of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ( email )

Shohaday-e-Gomnam Blvd.
Alem Sq.
Yazd
Iran

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
215
Abstract Views
1,142
rank
175,111
PlumX Metrics