Electrochemical Immunosensor Based on Antibody-Nanoparticle Hybrid for Specific Detection of the Dengue Virus NS1 Biomarker

Journal of The Electrochemical Society 163 (2016) B19-B25

7 Pages Posted: 16 Jan 2016

See all articles by Nadiya Darwish

Nadiya Darwish

University of Malaya (UM) - Department of Chemistry

Aoday Alrawi

University of Malaya (UM)

Shamala Sekaran

University of Malaya (UM)

Yatimah Alias

University of Malaya (UM) - Department of Chemistry

Sook Mei Khor

University of Malaya (UM) - Department of Chemistry

Date Written: January 7, 2016

Abstract

Dengue virus infection is a very deadly disease that threatens human lives in subtropical and tropical regions. Thus, for early and reliable diagnosis of Dengue virus infection, a sensitive, specific, and label-free electrochemical immunosensor was developed for the direct detection of the unstructured protein NS1. The NS1 biosensor was designed with a biosensing surface consisting of antifouling moieties and biorecognition molecules to enhance the specificity of the immunosensor for target analyte detection in complicated biological samples such as human sera. The immunosensor exhibited a wide detection range (5-4000 ng mL) based on electrochemical impedance spectroscopy (EIS) measurements with a coefficient of determination (R) of 0.94, correlation coefficient (R) of 0.95, high reproducibility, and good stability for 21 days at 4◦C. The developed immunosensor was able to detect the NS1 antigen in actual serum specimens from patients infected with Dengue virus. Moreover, the immunosensor is not only highly selective for the NS1 antigen, but also did not cross-react with human sera infected with malaria parasites.

Keywords: Electrochemical immunosensor, NS1 biomarker

JEL Classification: I1, I10

Suggested Citation

Darwish, Nadiya and Alrawi, Aoday and Sekaran, Shamala and Alias, Yatimah and Khor, Sook Mei, Electrochemical Immunosensor Based on Antibody-Nanoparticle Hybrid for Specific Detection of the Dengue Virus NS1 Biomarker (January 7, 2016). Journal of The Electrochemical Society 163 (2016) B19-B25. Available at SSRN: https://ssrn.com/abstract=2712126

Nadiya Darwish

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Aoday Alrawi

University of Malaya (UM) ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
University of Malaya (UM)
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Shamala Sekaran

University of Malaya (UM) ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
University of Malaya (UM)
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Yatimah Alias

University of Malaya (UM) - Department of Chemistry ( email )

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Sook Mei Khor (Contact Author)

University of Malaya (UM) - Department of Chemistry ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Register to save articles to
your library

Register

Paper statistics

Downloads
79
Abstract Views
341
rank
309,871
PlumX Metrics