A Simultaneous Derivatization of 3-Monochloropropanediol and 1,3-Dichloropropane with Hexamethyldisilazane–Trimethylsilyl Trifluoromethanesulfonate at Room Temperature for Efficient Analysis of Food Sample Analysis

10 Pages Posted: 25 Jan 2016

See all articles by Bai Lee

Bai Lee

University of Malaya (UM) - Department of Chemistry

Che Wan Jasimah Wan Mohamed Radzi

University of Malaya (UM), Science and Technology Studies Department

Sook Mei Khor

University of Malaya (UM) - Department of Chemistry

Date Written: January 21, 2016

Abstract

This paper reports the application of hexamethyldisilazane-trimethylsilyl trifluoromethanesulfonate (HMDS-TMSOTf) for the simultaneous silylation of 3-monochloro-1,2-propanediol (3-MCPD) and 1,3-dicholoropropanol (1,3-DCP) in solid and liquid food samples. 3-MCPD and 1,3-DCP are chloropropanols that have been established as Group 2B carcinogens in clinical testing. They can be found in heat-processed food, especially when an extended high-temperature treatment is required. However, the current AOAC detection method is time-consuming and expensive. Thus, HMDS-TMSOTf was used in this study to provide a safer, and cost-effective alternative to the HFBI method. Three important steps are involved in the quantification of 3-MCPD and 1,3-DCP: extraction, derivatization and quantification. The optimization of the derivatization process, which involved focusing on the catalyst volume, derivatization temperature, and derivatization time was performed based on the findings obtained from both the Box-Behnken modeling and a real experimental set up. With the optimized conditions, the newly developed method was used for actual food sample quantification and the results were compared with those obtained via the standard AOAC method. The developed method required less samples and reagents but it could be used to achieve lower limits of quantification (0.0043 mg L-1 for 1,3-DCP and 0.0011 mg L-1 for 3-MCPD) and detection (0.0028 mg L-1 for 1,3-DCP and 0.0008 mg L-1 for 3-MCPD). All the detected concentrations are below the maximum tolerable limit of 0.02 mg L-1. The percentage of recovery obtained from food sample analysis was between 83% and 96%. The new procedure was validated with the AOAC method and showed a comparable performance. The HMDS-TMSOTf derivatization strategy is capable of simultaneously derivatizing 1,3-DCP and 3-MCPD at room temperature, and it also serves as a rapid, sensitive, and accurate analytical method for food samples analysis.

Keywords: 3-Monochloropropanediol (3-MCPD), 1,3-Dichloropropanol (1,3-DCP), Acid hydrolyzed vegetable protein (acid–HVP), Silylation, Room temperature derivatization, Hexamethyldisilazane–trimethylsilyl trifluoromethanesulfonate (HMDS–TMSOTf)

JEL Classification: L50

Suggested Citation

Lee, Bai and Wan Mohamed Radzi, Che Wan Jasimah and Khor, Sook Mei, A Simultaneous Derivatization of 3-Monochloropropanediol and 1,3-Dichloropropane with Hexamethyldisilazane–Trimethylsilyl Trifluoromethanesulfonate at Room Temperature for Efficient Analysis of Food Sample Analysis (January 21, 2016). Available at SSRN: https://ssrn.com/abstract=2719996 or http://dx.doi.org/10.2139/ssrn.2719996

Bai Lee

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Che Wan Jasimah Wan Mohamed Radzi

University of Malaya (UM), Science and Technology Studies Department ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
University of Malaya (UM)
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Sook Mei Khor (Contact Author)

University of Malaya (UM) - Department of Chemistry ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Here is the Coronavirus
related research on SSRN

Paper statistics

Downloads
23
Abstract Views
361
PlumX Metrics