A Simple Egg Membrane Model for Understanding Diffusion Characteristics of Nanoparticles and Amino Acids

12 Pages Posted: 5 Jul 2017

See all articles by Deepika Tari

Deepika Tari

University of Mumbai

Shalaka Haryan

University of Mumbai

Kaustubh Patankar

University of Mumbai

Vinod Jaiswal

University of Mumbai

Mahesh Samant

University of Mumbai

S. Sivakami

University of Mumbai

P M Dongre

University of Mumbai

Date Written: May 24, 2016

Abstract

This study reports passive diffusion (in vitro) of silver nanoparticles with combination of aromatic amino acids tryptophan, phenylalanine, tyrosine across a biological membrane model. The experiments were carried out under physiological environment at pH 7.4. The chicken egg shell outer membrane model was used to study the passive diffusion of the above materials. The passive diffusion was performed against and towards gravitation for 24 and 48hrs. Fick’s first law of diffusion was adopted for quantification of diffusion coefficient, permeability constant and diffusion rate. The egg shell membrane was characterised using scanning electron microscopy (SEM). The silver nanoparticles were synthesized by chemical degradation route and characterised by UV visible spectroscopy and dynamic light scattering. An average size of nanoparticles obtained was 62nm. The diffusion rate of amino acids was higher than silver nanoparticles; however it was enhanced in presence of silver nanoparticles. Permeability coefficient and diffusion coefficient were higher in amino acids than silver nanoparticles. The possible mechanisms have been explained on the basis of molecular properties.

Keywords: Peptide, Folding, Beta Hairpin, Loop, Amino Acids, Vacccine Development, Chicken Egg Shell Membrane, Diffusion Rate, Permeability Constant, Silver Nanoparticles

Suggested Citation

Tari, Deepika and Haryan, Shalaka and Patankar, Kaustubh and Jaiswal, Vinod and Samant, Mahesh and Sivakami, S. and Dongre, P M, A Simple Egg Membrane Model for Understanding Diffusion Characteristics of Nanoparticles and Amino Acids (May 24, 2016). Available at SSRN: https://ssrn.com/abstract=2783837 or http://dx.doi.org/10.2139/ssrn.2783837

Deepika Tari

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

Shalaka Haryan

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

Kaustubh Patankar

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

Vinod Jaiswal

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

Mahesh Samant

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

S. Sivakami

University of Mumbai

Mumbai
Maharashtra
Mumbai, 400 019
India

P M Dongre (Contact Author)

University of Mumbai ( email )

Mumbai
Maharashtra
Mumbai, 400 019
India

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