Assessment of Various Pretreatment and Extraction Methods for the Extraction of Bioactive Compounds from Orthosiphon Stamineus Leaf via Microstructures Analysis

International Journal of Food Engineering, Vol. 12(7), p. 711-717, 2016

14 Pages Posted: 14 Mar 2017

See all articles by Tiam You See

Tiam You See

University of Malaya (UM) - Department of Chemical Engineering

S. I. Tee

University of Malaya (UM) - Department of Chemical Engineering

Teck Nam

The University of Auckland

Chung-Hung Chan

affiliation not provided to SSRN

Rozita Yusoff

University of Malaya (UM) - Department of Chemistry

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Date Written: February 22, 2016

Abstract

The impacts of various methods such as mechanical grinding, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and also sample pretreatments using acid and alkali on the microstructure of plant sample were studied for the extraction of bioactive compounds from Orthosiphon stamineus leaf. From scanning electron microscopy (SEM) analysis of the extracted sample, UAE and MAE induced significant disruption on glandular trichomes structure, which is the main site for biosynthesis of plant secondary metabolites. This improves the diffusion of bioactive compound and resulted in approximately 86-95% of the total extraction yield quantified by conventional Soxhlet extraction. Chemical pretreatments generally imparted weaker microstructures disruption thus slight improvement on the extraction yields was observed. In this case, acid reagent is more suitable for the pretreatment as the presence of alkali decomposes the bioactive compounds. In a nutshell, the performance of an extraction strongly depends on its degree of disruption on the plant sample microstructure.

Keywords: Bioactive Compounds, Microstructure, Extraction Systems, Ultrasonic-Assisted Extraction, Microwave-Assisted Extraction

JEL Classification: C61, A00, B00, C90, I20, Q20, Q00, Q16, Q32

Suggested Citation

See, Tiam You and Tee, S. I. and Ang, Teck Nam and Chan, Chung-Hung and Yusoff, Rozita and Ngoh, Gek-Cheng, Assessment of Various Pretreatment and Extraction Methods for the Extraction of Bioactive Compounds from Orthosiphon Stamineus Leaf via Microstructures Analysis (February 22, 2016). International Journal of Food Engineering, Vol. 12(7), p. 711-717, 2016, Available at SSRN: https://ssrn.com/abstract=2921729

Tiam You See

University of Malaya (UM) - Department of Chemical Engineering ( email )

Centre for Separation Science and Technology,
Faculty of Engineering
Kuala Lumpur, 50603
Malaysia

S. I. Tee

University of Malaya (UM) - Department of Chemical Engineering ( email )

Centre for Separation Science and Technology,
Faculty of Engineering
Kuala Lumpur, 50603
Malaysia

Teck Nam Ang

The University of Auckland ( email )

Department of Chemical and Materials Engineering
Auckland, AK 1010
New Zealand

Chung-Hung Chan (Contact Author)

affiliation not provided to SSRN

Rozita Yusoff

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

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