Effects of Nonionic Surfactants -Alkyl--Dimethylamine--Oxides on the Structure of a Phospholipid Bilayer. Small-Angle X-Ray Diffraction Study

Science Direct Working Paper No S1574-0331(04)70760-2

24 Pages Posted: 8 Jun 2017 Last revised: 20 Jan 2018

See all articles by J. Karlovská

J. Karlovská

Comenius University - Department of Physical Chemistry of Drugs, Faculty of Pharmacy

Karl Lohner

University of Vienna - Institute of Biophysics and X-ray Structure Research

Gábor Degovics

University of Vienna - Institute of Biophysics and X-ray Structure Research

Ivan Lacko

Comenius University - Department of Chemical Theory of Drugs, Faculty of Pharmacy

Ferdinand Devínsky

Univerzita Komenského v Bratislave

Pavol Balgavy

Comenius University - Department of Physical Chemistry of Drugs, Faculty of Pharmacy

Date Written: October 2003

Abstract

Effects of nonionic surfactants -alkyl--dimethylamine--oxides (CnNO, n is the number of alkyl carbons) on the structure of egg yolk phosphatidylcholine (EYPC) bilayers in the lamellar fluid phase was studied by small-angle X-ray diffraction as a function of HO:EYPC and CnNO:EYPC molar ratios. The bilayer thickness and the lipid surface area at the bilayer - aqueous interface were calculated from the repeat period of the lamellar phase, based on the model that water and EYPC+CnNO molecules form separated layers and that their molecular volumes are additive. In the studied range of =CnNO:EYPC molar ratios up to 1:1, and change linearly. The slopes and are equal to -0.876±0.027 nm and 0.347±0.006 nm for C6NO, -1.025±0.060 nm and 0.433±0.025 nm for C8NO, -0.836±0.046 nm and 0.405±0.018 nm for C10NO, -0.604±0.015 nm and 0.375±0.007 nm for C12NO, -0.279±0.031 nm and 0.318±0.005 nm for C14NO, -0.0865±0.070 nm and 0.2963±0.014 nm for C16NO, and -0.040±0.022 nm and 0.297±0.002 nm for C18NO, respectively, at full bilayer hydration. The peak-peak distance in the bilayer electron density profile, which relates to the P-P distance obtained from the first 4 diffraction peaks by the Fourier transform also depends linearly on , and the slope is -0.528±0.065 nm for C6NO, -0.680±0.018 nm for C8NO, -0.573±0.021 nm for C10NO, -0.369±0.075 nm for C12NO, -0.190±0.015 for C14NO, -0.088±0.016 nm for C16NO and -0.094±0.016 nm for C18NO. The effects of CnNO on and are the results of CnNO insertion into EYPC bilayers and depend on the hydrophobic mismatch between CnNO and EYPC hydrocarbon chains and on the lateral interactions of CnNO and EYPC in the bilayer.

Keywords: nonionic surfactants, -alkyl--dimethylamine--oxides, egg yolk phosphatidylcholine, bilayer, X-ray diffraction

Suggested Citation

Karlovská, J. and Lohner, Karl and Degovics, Gábor and Lacko, Ivan and Devínsky, Ferdinand and Balgavy, Pavol, Effects of Nonionic Surfactants -Alkyl--Dimethylamine--Oxides on the Structure of a Phospholipid Bilayer. Small-Angle X-Ray Diffraction Study (October 2003). Science Direct Working Paper No S1574-0331(04)70760-2, Available at SSRN: https://ssrn.com/abstract=2981186

J. Karlovská

Comenius University - Department of Physical Chemistry of Drugs, Faculty of Pharmacy

Odboja ´rov 10
Bratislava, SK-832 32
Slovakia

Karl Lohner

University of Vienna - Institute of Biophysics and X-ray Structure Research

A-8042 Graz
Schmiedlstrasse 6
Australia

Gábor Degovics

University of Vienna - Institute of Biophysics and X-ray Structure Research

A-8042 Graz
Schmiedlstrasse 6
Australia

Ivan Lacko

Comenius University - Department of Chemical Theory of Drugs, Faculty of Pharmacy

Odbojarov 2
83232 Bratislava
Slovakia

Ferdinand Devínsky

Univerzita Komenského v Bratislave ( email )

Slovakia

Pavol Balgavy (Contact Author)

Comenius University - Department of Physical Chemistry of Drugs, Faculty of Pharmacy ( email )

Odboja ´rov 10
Bratislava, SK-832 32
Slovakia

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