Hypoglycaemia and Improved Testicular Parameters in Sesamum Radiatum Treated Normo-Glycaemic Adult Male Sprague Dawley Rats

African Journal of Biotechnology Vol. 8 (12), 2009

Posted: 16 Aug 2017 Last revised: 20 Aug 2017

See all articles by Lukeman Shittu

Lukeman Shittu

Jireh Laboratories International LLC; Houston Community College, Department of Biology; University of Abuja, Department of Anatomical Sciences; Benue State University, Department of Anatomy; Lagos State University - Department of Anatomy

Remilekun Shittu

Bolomedics Laboratories

Ogundipe Olufemi

Nottinghamshire Healthcare Nhs Trust - Queens Medical Center

Adetokunbo Tayo

Lagos University Teaching Hospital

O.A. Ashiru

Lagos State University - Department of Anatomy

Date Written: June 1, 2009

Abstract

The development of a new dietary adjunct with a novel natural antioxidant impact on diabetes mellitus with prevention of its long term deleterious effect on the male fertility in general has been increasingly expressed in recent time. Hence, we aim to evaluate the effects of aqueous extract of Sesame radiatum leaves on adult male Sprague Dawley rats’ testis using unbiased stereological, biochemical and hormonal studies. Thirty adult male rats were divided into three groups of 10 rats each. The treated groups; 1 and 2 received 28.0 and 14 mg/kg bwt of aqueous extract of sesame leaves via oral garvage, respectively, while the control group received equal volume of 0.9% (w/v) normal saline per day for 6 weeks. Serum follicle-stimulating hormone (FSH), testosterone and blood glucose were assayed. In addition five microns of uniformly random transverse sections of processed testicular tissues were equally analyzed using an un-biased stereological study. The result showed that the mean percentage volume fractions (Vf) of epithelial cells and lumen of the testis were 76% (P<0.05) and 22% (P<0.05), respectively, in the low dose compared to control. Conversely to the Vf of stroma and glucose level in treated, the mean raw weights of testis and of body weights (g) with the Vf of ST-epithelium in the treated groups were found (P>0.05) higher than the control in a dose related manner. Serum testosterone and FSH were significantly higher and lower, respectively, in the high dose sesame when compared to control. Sesame leaves intake improved glucose profile and testicular parameters in a dose related manner via possible improved insulin activity on the cells with a stimulatory impact on sperm production. This also confirmed its folkloric claims.

Keywords: Sesame leaves, Phytoestrogen lignans, SD rats, Hypoglycemia, Normoglycemic rats, Epithelial hyperplasia, Stereology

JEL Classification: I10

Suggested Citation

Shittu, Lukeman and Shittu, Remilekun and Olufemi, Ogundipe and Tayo, Adetokunbo and Ashiru, O.A., Hypoglycaemia and Improved Testicular Parameters in Sesamum Radiatum Treated Normo-Glycaemic Adult Male Sprague Dawley Rats (June 1, 2009). African Journal of Biotechnology Vol. 8 (12), 2009. Available at SSRN: https://ssrn.com/abstract=3017018

Lukeman Shittu (Contact Author)

Jireh Laboratories International LLC ( email )

P.O.BOX 1813
ALIEF, TX HOUSTON 77411
United States
346-322-5042 (Phone)

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Houston Community College, Department of Biology ( email )

3100 Main Street
Houston, TX Harris County 77002
United States

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

University of Abuja, Department of Anatomical Sciences ( email )

Airport Road Giri
Gwagalada
F.C.T, West Africa 00234
Nigeria

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Benue State University, Department of Anatomy ( email )

Along Gboko Rd, P.M.B.102119 Makurdi-Gboko Rd
Makurdi, 97001
Nigeria

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Lagos State University - Department of Anatomy ( email )

Ikeja, Lagos
Nigeria

Remilekun Shittu

Bolomedics Laboratories ( email )

Egbeda, Lagos
Nigeria

Ogundipe Olufemi

Nottinghamshire Healthcare Nhs Trust - Queens Medical Center

Duncan MacMillan House, Porchester Rd
Nottingham, NG3 6AA
United Kingdom

Adetokunbo Tayo

Lagos University Teaching Hospital ( email )

Idi-Araba,Surulere
Lagos, PMB 12003
Nigeria

O.A. Ashiru

Lagos State University - Department of Anatomy

Ikeja, Lagos
Nigeria

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