Stereological Evidences of Epithelial Hypoplasia of Seminiferous Tubules Induced by Mesterolone in Adult Sprague-Dawley Rats

African Journal of Endocrinology and Metabolism Vol.7 No.1 July - December 2008.

4 Pages Posted: 11 Aug 2017

See all articles by Lukeman Shittu

Lukeman Shittu

Jireh Laboratories International LLC; Houston Community College, Department of Biology; University of Abuja, Department of Anatomical Sciences; Benue State University, Department of Anatomy; Lagos State University - Department of Anatomy

Remilekun Shittu

Bolomedics Laboratories

A.A. Osinubi

Lagos State University - Department of Anatomy

O.A. Ashiru

Lagos State University - Department of Anatomy

Date Written: 2008

Abstract

Background: Anabolic-androgenic steroid compounds are one of the most widely abused drugs by athletes and muscle builders with the goal of improving performance/ability, appearance, or muscle mass. In addition, these steroids are widely used in the treatment of male infertility and subfertility. However, increasing concern has been shown that these compounds may not only offer unappreciable benefits to infertile and subfertile males, but might have deleterious effects on both human and animal physiology and sperm quality. There is a dearth of knowledge on the structural and quantitative changes of the testis secondary to this group of compounds.

Objective: The present study was carried out to evaluate the effects of mesterolone (proviron), an anabolic-androgenic steroid, on some of the histomorphometric and stereological parameters of the seminiferous tubules in Sprague-Dawley rat.

Materials and Methods: Two groups of 10 adult male rats were used. The treated group was given 0.06 mg/kg body weight/day of mesterolone by gavage for six weeks while the control group received equal volume of 0.9% normal saline per day. Five µm of uniformly random serial sections of the processed testicular tissues were analyzed using un-biased stereological and histomorphometric studies.

Results: The results showed that the percentage mean volume density of both the tubular lumen and epithelial height increased by 35% (p< 0.05) and decreased by 50% (p<0.05), respectively compared to the control. Mesterolone also caused a significant decline in sperm concentration.

Conclusion: Mesterolone produces epithelial hypoplasia in the testis post continuous management.

Keywords: Testis, Stereology, Mesterolone (Proviron, Seminiferous tubules)

JEL Classification: I10

Suggested Citation

Shittu, Lukeman and Shittu, Remilekun and Osinubi, A.A. and Ashiru, O.A., Stereological Evidences of Epithelial Hypoplasia of Seminiferous Tubules Induced by Mesterolone in Adult Sprague-Dawley Rats (2008). African Journal of Endocrinology and Metabolism Vol.7 No.1 July - December 2008.. Available at SSRN: https://ssrn.com/abstract=3017024

Lukeman Shittu (Contact Author)

Jireh Laboratories International LLC ( email )

P.O.BOX 1813
ALIEF, TX HOUSTON 77411
United States
346-322-5042 (Phone)

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Houston Community College, Department of Biology ( email )

3100 Main Street
Houston, TX Harris County 77002
United States

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

University of Abuja, Department of Anatomical Sciences ( email )

Airport Road Giri
Gwagalada
F.C.T, West Africa 00234
Nigeria

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Benue State University, Department of Anatomy ( email )

Along Gboko Rd, P.M.B.102119 Makurdi-Gboko Rd
Makurdi, 97001
Nigeria

HOME PAGE: http://linkedin.com/in/drlukemanjosephshittu

Lagos State University - Department of Anatomy ( email )

Ikeja, Lagos
Nigeria

Remilekun Shittu

Bolomedics Laboratories ( email )

Egbeda, Lagos
Nigeria

A.A. Osinubi

Lagos State University - Department of Anatomy

Ikeja, Lagos
Nigeria

O.A. Ashiru

Lagos State University - Department of Anatomy

Ikeja, Lagos
Nigeria

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