puc-header

Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelial mitosis

39 Pages Posted: 4 Apr 2018 Sneak Peek Status: Review Complete

See all articles by Alexander Nestor-Bergmann

Alexander Nestor-Bergmann

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

Georgina A. Stooke-Vaughan

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

Georgina K. Goddard

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

Tobias Starborg

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

Oliver E. Jensen

University of Manchester - School of Mathematics

Sarah Woolner

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

More...

Abstract

Distinct mechanisms involving cell shape and mechanical force are known to influence the rate and orientation of division in cultured cells. However, uncoupling the impact of shape and force in tissues remains challenging. Combining stretching of Xenopus laevis tissue with a novel method of inferring relative mechanical stress, we find separate roles for cell shape in orientating division and mechanical stress in cueing division. We demonstrate that division orientation is best predicted by an axis of cell shape defined by the position of tricellular junctions, which aligns exactly with the principal axis of local cell stress rather than the tissue-level stress. The alignment of division to cell shape requires functional cadherin, but is not sensitive to relative cell stress magnitude. In contrast, cell proliferation rate is more directly regulated by mechanical stress, being correlated with relative isotropic stress, and can be decoupled from cell shape when myosin II is depleted.

Suggested Citation

Nestor-Bergmann, Alexander and Stooke-Vaughan, Georgina A. and Goddard, Georgina K. and Starborg, Tobias and Jensen, Oliver E. and Woolner, Sarah, Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelial mitosis (2018). Available at SSRN: https://ssrn.com/abstract=3155547 or http://dx.doi.org/10.2139/ssrn.3155547
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Alexander Nestor-Bergmann

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research ( email )

B.3016 Michael Smith Building
Oxford Road
Manchester, M13 9PT
United Kingdom

Georgina A. Stooke-Vaughan

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

B.3016 Michael Smith Building
Oxford Road
Manchester, M13 9PT
United Kingdom

Georgina K. Goddard

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

B.3016 Michael Smith Building
Oxford Road
Manchester, M13 9PT
United Kingdom

Tobias Starborg

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research

B.3016 Michael Smith Building
Oxford Road
Manchester, M13 9PT
United Kingdom

Oliver E. Jensen

University of Manchester - School of Mathematics

United Kingdom

Sarah Woolner (Contact Author)

University of Manchester - Wellcome Trust Centre for Cell-Matrix Research ( email )

B.3016 Michael Smith Building
Oxford Road
Manchester, M13 9PT
United Kingdom

Click here to go to Cell.com

Go to Cell.com

Paper statistics

Abstract Views
231
Downloads
11