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A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain Cell Type Targeting and Functionality

85 Pages Posted: 5 Apr 2018 Sneak Peek Status: Published

See all articles by Tanya L. Daigle

Tanya L. Daigle

Allen Institute for Brain Science

Linda Madisen

Allen Institute for Brain Science

Travis A. Hage

Allen Institute for Brain Science

Matthew T. Valley

Allen Institute for Brain Science

Ulf Knoblich

Allen Institute for Brain Science

Rylan S. Larsen

Allen Institute for Brain Science

Marc M. Takeno

Allen Institute for Brain Science

Lawrence Huang

Allen Institute for Brain Science

Hong Gu

Allen Institute for Brain Science

Rachael Larsen

Allen Institute for Brain Science

Maya Mills

Allen Institute for Brain Science

Alice Bosma-Moody

Allen Institute for Brain Science

La'Akea Siverts

Allen Institute for Brain Science

Miranda Walker

Allen Institute for Brain Science

Lucas T. Graybuck

Allen Institute for Brain Science

Zizhen Yao

Allen Institute for Brain Science

Olivia Fong

Allen Institute for Brain Science

Emma Garren

Allen Institute for Brain Science

Garreck Lenz

Allen Institute for Brain Science

Mariya Chavarha

Stanford University - Department of Neurobiology; Stanford University - Department of Bioengineering

Julie Pendergraft

Allen Institute for Brain Science

James Harrington

Allen Institute for Brain Science

Karla E. Hirokawa

Allen Institute for Brain Science

Julie A. Harris

Allen Institute for Brain Science

Medea McGraw

Allen Institute for Brain Science

Douglas R. Ollerenshaw

Allen Institute for Brain Science

Kimberly Smith

Allen Institute for Brain Science

Christopher A. Baker

Allen Institute for Brain Science

Jonathan T. Ting

Allen Institute for Brain Science - Human Cell Types

Susan M. Sunkin

Allen Institute for Brain Science

Jerome Lecoq

Allen Institute for Brain Science

Michael Z. Lin

Stanford University - Department of Neurobiology; Stanford University - Department of Bioengineering

Edward S. Boyden

Massachusetts Institute of Technology (MIT) - MIT Media Laboratory; Massachusetts Institute of Technology (MIT) - McGovern Institute

Gabe J. Murphy

Allen Institute for Brain Science

Nuno da Costa

Allen Institute for Brain Science

Jack Waters

Allen Institute for Brain Science

Lu Li

Allen Institute for Brain Science

Bosiljka Tasic

Allen Institute for Brain Science

Hongkui Zeng

Allen Institute for Brain Science

More...

Abstract

Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating the study of their function. We here report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically-defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of highprecision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

Suggested Citation

Daigle, Tanya L. and Madisen, Linda and Hage, Travis A. and Valley, Matthew T. and Knoblich, Ulf and Larsen, Rylan S. and Takeno, Marc M. and Huang, Lawrence and Gu, Hong and Larsen, Rachael and Mills, Maya and Bosma-Moody, Alice and Siverts, La'Akea and Walker, Miranda and Graybuck, Lucas T. and Yao, Zizhen and Fong, Olivia and Garren, Emma and Lenz, Garreck and Chavarha, Mariya and Pendergraft, Julie and Harrington, James and Hirokawa, Karla E. and Harris, Julie A. and McGraw, Medea and Ollerenshaw, Douglas R. and Smith, Kimberly and Baker, Christopher A. and Ting, Jonathan T. and Sunkin, Susan M. and Lecoq, Jerome and Lin, Michael Z. and Boyden, Edward S. and Murphy, Gabe J. and da Costa, Nuno and Waters, Jack and Li, Lu and Tasic, Bosiljka and Zeng, Hongkui, A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain Cell Type Targeting and Functionality (2018). Available at SSRN: https://ssrn.com/abstract=3155578 or http://dx.doi.org/10.2139/ssrn.3155578
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Tanya L. Daigle

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Linda Madisen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Travis A. Hage

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Matthew T. Valley

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Ulf Knoblich

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Rylan S. Larsen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Marc M. Takeno

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Lawrence Huang

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Hong Gu

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Rachael Larsen

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Maya Mills

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Alice Bosma-Moody

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

La'Akea Siverts

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Miranda Walker

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Lucas T. Graybuck

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Zizhen Yao

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Olivia Fong

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Emma Garren

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Garreck Lenz

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Mariya Chavarha

Stanford University - Department of Neurobiology

Stanford, CA 94305
United States

Stanford University - Department of Bioengineering

Stanford, CA 94305
United States

Julie Pendergraft

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

James Harrington

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Karla E. Hirokawa

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Julie A. Harris

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Medea McGraw

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Douglas R. Ollerenshaw

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Kimberly Smith

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Christopher A. Baker

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Jonathan T. Ting

Allen Institute for Brain Science - Human Cell Types

United States

Susan M. Sunkin

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Jerome Lecoq

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Michael Z. Lin

Stanford University - Department of Neurobiology

Stanford, CA 94305
United States

Stanford University - Department of Bioengineering

Stanford, CA 94305
United States

Edward S. Boyden

Massachusetts Institute of Technology (MIT) - MIT Media Laboratory

20 Ames St.
Cambridge, MA 02139-4307
United States

Massachusetts Institute of Technology (MIT) - McGovern Institute

77 Massachusetts Avenue
50 Memorial Drive
Cambridge, MA 02139-4307
United States

Gabe J. Murphy

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Nuno Da Costa

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Jack Waters

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Lu Li

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Bosiljka Tasic

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

Hongkui Zeng (Contact Author)

Allen Institute for Brain Science

615 Westlake Ave N
Seattle, WA 98109
United States

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