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Mechanical Energy of Protein Phase Separation Shapes Cell Membranes

72 Pages Posted: 12 Apr 2018 Sneak Peek Status: Review Complete

See all articles by Louis-Philippe Bergeron-Sandoval

Louis-Philippe Bergeron-Sandoval

University of Montreal, Faculty of Medicine, Department of Biochemistry and Molecular Medicine

Hossein Khadivi Heris

McGill University, Faculty of Engineering, Department of Bioengineering

Adam Hendricks

McGill University, Faculty of Engineering, Department of Bioengineering

Allen Ehrlicher

McGill University, Faculty of Engineering, Department of Bioengineering

Paul François

McGill University - Department of Physics; McGill University - Integrated Quantitative Biology Initiative

Rohit Pappu

Washington University in St. Louis, School of Engineering & Applied Science, Center for Biological Systems Engineering ; Washington University in St. Louis - Department of Biomedical Engineering

Stephen W. Michnick

University of Montreal - Department of Biochemistry and Molecular Medicine

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Abstract

Clathrin-mediated endocytosis (CME) underlies intra- and extracellular material trafficking in eukaryotes, and is essential to protein metabolism, intercellular signaling, membrane remodeling and other cell regulatory processes. Although CME is usually driven by F-actin polymerization, membrane invagination can also occur through actin independent mechanisms. Here, we show that viscoelastic protein condensates that form via liquid-liquid phase separation at the sites of endocytosis initiation facilitate actin independent CME. Binding energies of the condensate with the membrane and surrounding cytosol provides work required to drive membrane invagination. Our findings expand the repertoire of functions associated with protein condensates that form via liquid-liquid phase separation to include their ability to do work at soft interfaces, thus shaping and organizing cellular matter.

Suggested Citation

Bergeron-Sandoval, Louis-Philippe and Heris, Hossein Khadivi and Hendricks, Adam and Ehrlicher, Allen and François, Paul and Pappu, Rohit and Michnick, Stephen W., Mechanical Energy of Protein Phase Separation Shapes Cell Membranes (2018). Available at SSRN: https://ssrn.com/abstract=3155762 or http://dx.doi.org/10.2139/ssrn.3155762
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Louis-Philippe Bergeron-Sandoval

University of Montreal, Faculty of Medicine, Department of Biochemistry and Molecular Medicine ( email )

Montreal
Canada

Hossein Khadivi Heris

McGill University, Faculty of Engineering, Department of Bioengineering

1001 Sherbrooke St. W
Montreal
Canada

Adam Hendricks

McGill University, Faculty of Engineering, Department of Bioengineering ( email )

1001 Sherbrooke St. W
Montreal
Canada

Allen Ehrlicher

McGill University, Faculty of Engineering, Department of Bioengineering ( email )

1001 Sherbrooke St. W
Montreal
Canada

Paul François

McGill University - Department of Physics ( email )

1001 Sherbrooke St. W
Montreal
Canada

McGill University - Integrated Quantitative Biology Initiative ( email )

Montreal
Canada

Rohit Pappu

Washington University in St. Louis, School of Engineering & Applied Science, Center for Biological Systems Engineering ( email )

One Brookings Drive
St. Louis, MO
United States

Washington University in St. Louis - Department of Biomedical Engineering ( email )

One Brookings Drive
Campus Box 1208
Saint Louis, MO 63130-4899
United States

Stephen W. Michnick (Contact Author)

University of Montreal - Department of Biochemistry and Molecular Medicine ( email )

2900 Boulevard Edouard-Montpetit
Montréal, Québec H3T 1J4
Canada

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