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Local Nucleation of Microtubule Bundles Through Tubulin Concentration Into a Condensed Tau Phase

32 Pages Posted: 8 Apr 2018 Sneak Peek Status: Published

See all articles by Amayra Hernández-Vega

Amayra Hernández-Vega

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Marcus Braun

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Lara Scharrel

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Marcus Jahnel

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Susanne Wegmann

Harvard University - Department of Neurology

Bradley T. Hyman

Harvard University - Department of Neurology

Simon Albert

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Stefan Diez

Dresden University of Technology - B CUBE – Center for Molecular Bioengineering

Anthony A. Hyman

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

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Abstract

Non‐centrosomal microtubule bundles play important roles in cellular organization and function. Although many diverse proteins are known that can bundle microtubules, biochemical mechanisms by which cells could locally control the nucleation and formation of microtubule bundles are understudied. Here, we demonstrate that concentration of tubulin into a condensed, liquid‐like compartment composed of the unstructured neuronal protein tau is sufficient to nucleate microtubule bundles. We show that under conditions of macro‐molecular crowding, tau forms liquid drops. Tubulin partitions into these drops, efficiently increasing tubulin concentration and driving the nucleation of microtubules. These growing microtubules form bundles enclosed in a liquid sheath of tau. Our data suggest that condensed tau compartments could promote the formation of microtubule bundles in neurons by acting as non ‐ centrosomal microtubule nucleation centers and that liquid‐like tau encapsulation could provide both stability and plasticity to long axonal microtubule bundles.

Suggested Citation

Hernández-Vega, Amayra and Braun, Marcus and Scharrel, Lara and Jahnel, Marcus and Wegmann, Susanne and Hyman, Bradley T. and Albert, Simon and Diez, Stefan and Hyman, Anthony A., Local Nucleation of Microtubule Bundles Through Tubulin Concentration Into a Condensed Tau Phase (2018). Available at SSRN: https://ssrn.com/abstract=3155795 or http://dx.doi.org/10.2139/ssrn.3155795
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Amayra Hernández-Vega

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Pfotenhauerstr. 108
Dresden, 01307
Germany

Marcus Braun

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Pfotenhauerstr. 108
Dresden, 01307
Germany

Lara Scharrel

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Pfotenhauerstr. 108
Dresden, 01307
Germany

Marcus Jahnel

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Pfotenhauerstr. 108
Dresden, 01307
Germany

Susanne Wegmann

Harvard University - Department of Neurology

Wang Ambulatory Care Center, 8th Floor, Suite 835
Charlestown, MA 02129
United States

Bradley T. Hyman

Harvard University - Department of Neurology

Wang Ambulatory Care Center, 8th Floor, Suite 835
Charlestown, MA 02129
United States

Simon Albert

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Pfotenhauerstr. 108
Dresden, 01307
Germany

Stefan Diez

Dresden University of Technology - B CUBE – Center for Molecular Bioengineering ( email )

Arnoldstrasse 18
Dresden, 01307
Germany

Anthony A. Hyman (Contact Author)

Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) ( email )

Pfotenhauerstr. 108
Dresden, 01307
Germany

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