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Sustained Photosynthesis in Tobacco Leaves by Fast CO2-Fixing Enzymes Encapsulated in Micro-Compartments

57 Pages Posted: 6 Apr 2018 Sneak Peek Status: Review Complete

See all articles by Benedict M. Long

Benedict M. Long

Realizing Increased Photosynthetic Efficiency (RIPE)

Wei Yih Hee

Realizing Increased Photosynthetic Efficiency (RIPE)

Robert E. Sharwood

ARC Centre of Excellence for Translational Photosynthesis

Benjamin D. Rae

ARC Centre of Excellence for Translational Photosynthesis

Sarah Kaines

Realizing Increased Photosynthetic Efficiency (RIPE)

Yi-Leen Lim

Realizing Increased Photosynthetic Efficiency (RIPE)

Nghiem D. Nguyen

ARC Centre of Excellence for Translational Photosynthesis

Baxter Massey

Realizing Increased Photosynthetic Efficiency (RIPE)

Soumi Bala

ARC Centre of Excellence for Translational Photosynthesis

Susanne von Caemmerer

Realizing Increased Photosynthetic Efficiency (RIPE)

Murray R. Badger

Realizing Increased Photosynthetic Efficiency (RIPE)

G. Dean Price

Realizing Increased Photosynthetic Efficiency (RIPE)

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Abstract

Improvement of yield potential in global food crops has hit a breeding road-block which could be solved through genetic engineering. One promising strategy is the introduction of cyanobacterial CO2-concentrating-mechanisms (CCMs) into plant chloroplasts to enhance photosynthesis and yield. CCMs actively accumulate CO2 within specialized microcompartments called carboxysomes, where the enzyme Rubisco rapidly fixes CO2. Here we replaced the endogenous Rubisco large subunit gene with cyanobacterial Form-1A Rubisco and key structural proteins of α- carboxysomes in tobacco chloroplasts. Transformed plants produced purifiable carboxysomes, isometric with those of the source organism Cyanobium marinum. Carboxysomes encapsulated the introduced Rubisco and enabled autotrophic growth at elevated CO2. Our results demonstrate the formation of α-carboxysomes from a minimal gene set, and that cyanobacterial Form-1A Rubisco supports C3 photosynthesis at high CO2. This major advance informs the step-wise construction of fully-functional α-carboxysomes in chloroplasts which, along with other CCM components, are expected to improve crop plant performance.

Suggested Citation

Long, Benedict M. and Hee, Wei Yih and Sharwood, Robert E. and Rae, Benjamin D. and Kaines, Sarah and Lim, Yi-Leen and Nguyen, Nghiem D. and Massey, Baxter and Bala, Soumi and Caemmerer, Susanne von and Badger, Murray R. and Price, G. Dean, Sustained Photosynthesis in Tobacco Leaves by Fast CO2-Fixing Enzymes Encapsulated in Micro-Compartments (2018). Available at SSRN: https://ssrn.com/abstract=3155912 or http://dx.doi.org/10.2139/ssrn.3155912
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Benedict M. Long (Contact Author)

Realizing Increased Photosynthetic Efficiency (RIPE)

Wei Yih Hee

Realizing Increased Photosynthetic Efficiency (RIPE)

Robert E. Sharwood

ARC Centre of Excellence for Translational Photosynthesis

Benjamin D. Rae

ARC Centre of Excellence for Translational Photosynthesis

Sarah Kaines

Realizing Increased Photosynthetic Efficiency (RIPE)

Yi-Leen Lim

Realizing Increased Photosynthetic Efficiency (RIPE)

Nghiem D. Nguyen

ARC Centre of Excellence for Translational Photosynthesis

Baxter Massey

Realizing Increased Photosynthetic Efficiency (RIPE)

Soumi Bala

ARC Centre of Excellence for Translational Photosynthesis

Susanne von Caemmerer

Realizing Increased Photosynthetic Efficiency (RIPE)

Murray R. Badger

Realizing Increased Photosynthetic Efficiency (RIPE)

G. Dean Price

Realizing Increased Photosynthetic Efficiency (RIPE)

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