Advantages and Drawbacks of Next Generation Sequencing

5 Pages Posted: 5 Jun 2018

Date Written: February 5, 2018

Abstract

Starting in 1990 the Human Genome Project (HGP) allowed the sequencing of the whole human genome by 2003 (Gibson and Muse, 2009). The Sanger sequencing technique is a gold standard for sequencing DNA and was instrumental for HGP (Gibson and Muse, 2009). Unfortunately, Sanger sequencing involves much time and money to perform, and throughout the decades other methods of sequencing have been developed to reduce time and cost for sequencing large scale projects. In addition to time and cost of sequencing, hurdles have arisen — such as correct sequence assembly of repeated regions of DNA and how to navigate through mega-datasets. In this essay throughput, cost and other technological and biological aspects will be summarized for the next generation of sequencing technologies.

Keywords: Human Genome Project, DNA, Sequencing, Sanger

Suggested Citation

Cottrell, Paul, Advantages and Drawbacks of Next Generation Sequencing (February 5, 2018). Available at SSRN: https://ssrn.com/abstract=3183340 or http://dx.doi.org/10.2139/ssrn.3183340

Paul Cottrell (Contact Author)

Harvard University, Students ( email )

Cambridge, MA
United States

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