Diversity Analysis of Beta-Lactamase Among Uropathogenic Enterobacteriaceae
4 Pages Posted: 10 Jun 2018
Date Written: February 7, 2018
Cephalosporin resistance, particularly due to extended spectrum of Beta-lactamase among Enterobacteriaceae members is an increasing public health problem in India. Extended Spectrum of Beta-lactamases (ESBLs) are mainly coded for by three important gene classes namely blaTEM, blaSHV and blaCTX-M. The infections caused by these ESBL strains are widely prevalent in different geographical areas, and their genetic variants are extensive and change continuously.The present study focuses on determination of genetic diversity of ESBL among 75 pathogenic E.coli and Klebsiella sp isolated from urinary tract infected patients. Their resistance profile against Cephalosporin’s was determined and isolates presenting resistance to 80% of the cephalosporins tested were analyzed for phenotypes. The genotypic characterization of ESBLs was carried by PCR using the primers designed for NDM,TEM, SHV and CTX genotypes. The amplicons were further sequenced for confirmation of the ESBL types. The DNA amplicons sequence analyzed for the confirmation of the ESBL types. Sequence analysis was carried using Clustal W/Mega software and Phylogenetic tree was constructed. A boot value of 1000 was used for constructing tree by neighbor joining method. The diversity and variation of the sample at the active site was analyzed. The prevalence of SHV genotype was highest followed by TEM and CTX groups.
Keywords: Uropathogens, Escherichia coli, Klebsiella sp, ESBL
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