Development of an Analytical Method of Organochlorine Pesticide Residues in Bivalves from Oualidia in Morocco

7 Pages Posted: 4 Jun 2018 Last revised: 22 Jun 2018

See all articles by Fatima Habti

Fatima Habti

University Hassan II of Casablanca - Department of Chemistry

Soumia Belouafa

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis

A Bennamara

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis

Abdelmjid Abourriche

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis

Mustapha Tarhy

Moroccan Laboratory of Agriculture (LABOMAG) - Department of Organic Pollutants

Noureddine Fatini

Moroccan Laboratory of Agriculture (LABOMAG) - Department of Organic Pollutants

Date Written: May 28, 2018

Abstract

The preparation of samples for the analysis of persistent organic pollutants in bivalves in general and mussels in particular requires complex extraction methods for which it is often necessary to resort to several compelling preparation steps to be carried out. The QUEChERS method (Quick, Easy, Cheap, Effective, Rugged and Safe) is a softer alternative method than conventional extractions / purifications and leads to a less demanding implementation, as well as the use of less solvent. This preparation technique was adapted and evaluated for multiresidue analysis of organochlorine pesticides in "mussel" bivaves by gas chromatography coupled with tandem mass spectrometry (GC-MS / MS). The method was developed in two stages. In the first place, an optimization of the extraction step the extract is put in the freezer overnight complete to reduce the majority of the fat the second step was the optimization of the purification step. Different adsorbents were tested in direct or dispersed solid phase extraction mode. It emerges from this study that the best results were obtained by extraction with acetonitrile followed by a freezing step and finally purification by a mixture of adsorbents (MgSO4 LC-18 PSA). In addition, to demonstrate that the method is reliable, different validation parameters have been studied: linearity, quantification limit, recovery rates and selectivity. The results showed that, thanks to the changes introduced, it was possible to obtain a more selective method that improves the accuracy of about 13 organochlorine pesticides selected from the original method. It has been found that the method is suitable for the analysis of 13 POCs. In addition, with the developed method, recoveries between 70 and 120% and a relative standard deviation (RSDs) less than 20% were found.

Keywords: QuECHERS, organochlorine pesticide residues, bivalves, development, validation, GC-MS/MS.

Suggested Citation

Habti, Fatima and Belouafa, Soumia and Bennamara, A and Abourriche, Abdelmjid and Tarhy, Mustapha and Fatini, Noureddine, Development of an Analytical Method of Organochlorine Pesticide Residues in Bivalves from Oualidia in Morocco (May 28, 2018). Smart Application and Data Analysis for Smart Cities (SADASC'18). Available at SSRN: https://ssrn.com/abstract=3186341 or http://dx.doi.org/10.2139/ssrn.3186341

Fatima Habti (Contact Author)

University Hassan II of Casablanca - Department of Chemistry ( email )

Casablanca
Morocco

Soumia Belouafa

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis ( email )

Casablanca
Morocco

A Bennamara

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis ( email )

Casablanca
Morocco

Abdelmjid Abourriche

University Hassan II of Casablanca - Laboratory of Biomolecules and Organic Synthesis ( email )

Casablanca
Morocco

Mustapha Tarhy

Moroccan Laboratory of Agriculture (LABOMAG) - Department of Organic Pollutants ( email )

Casablanca
Morocco

Noureddine Fatini

Moroccan Laboratory of Agriculture (LABOMAG) - Department of Organic Pollutants ( email )

Casablanca
Morocco

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