Identification of Isozyme and Molecular Markers Linked to Leaf Rust Resistance in New Egyptian Wheat Lines

8 Pages Posted: 11 Jul 2018

See all articles by Ramadan M. Esmail

Ramadan M. Esmail

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Abdel Sattar A. Abdel Sattar

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Mohamed A. Abou-Ellail

University of Aswan, Faculty of Agriculture and Natural Resources, Department of Genetics

Heba A. Mahfouze

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Hoda M.A. Waziri

Plant Pathology Research Institute - Virus and Phytoplasma Department

Sherin A. Mahfouze

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Date Written: June 23, 2018

Abstract

Leaf rust is a crucial disease of wheat (Triticum aestivium L.) in Egypt and worldwide. It causes by fungi Puccinia triticina. Leaf rust could be controlled by genetic resistance to limit yield damages. In this study, we evaluate new wheat lines for resistance to leaf rust disease. In addition, determine the activities of polyphenol oxidase and peroxidase in leaf rust resistance wheat lines compared with the healthy control. Also, we identify Inter-simple sequence repeat (ISSR-PCR) markers linked to Lr resistant genes at the seedling stage. All wheat lines were resistant to leaf rust at seedling stage. However, under the field conditions three lines No. 3, 7 and 8 were resistant, while other seven were susceptible. Consequently, it could be concluded that lines No. 3, 7 and 8 were resistant to leaf rust in both stages. Eight anchored ISSR primers were screened to identify polymorphic bands between the leaf rust resistance and control wheat plants. A total of 104 scorable fragments were amplified of which 54 monomorphic and 46 bands were polymorphic resulting in a polymorphism of 44.23%. The largest number of ISSR-PCR specific markers scored in leaf rust resistance wheat line No. 10 (three markers), followed by leaf rust resistance wheat lines No. 2, 3, 4 and 8 (two). The lowest number of specific bands existed in resistance line No. 1 (one unique marker). These markers could be applied in wheat breeding programs, as marker assisted selection. By a backcross program was achieved by recurrent parents between the susceptible high-quality wheat and the resistant gene carrying ones as donor parents.

Suggested Citation

Esmail, Ramadan M. and Abdel Sattar, Abdel Sattar A. and Abou-Ellail, Mohamed A. and Mahfouze, Heba A. and Waziri, Hoda M.A. and Mahfouze, Sherin A., Identification of Isozyme and Molecular Markers Linked to Leaf Rust Resistance in New Egyptian Wheat Lines (June 23, 2018). Applied Science Reports, Vol. 19, No. 1, 2017, Available at SSRN: https://ssrn.com/abstract=3201414

Ramadan M. Esmail

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Egypt

Abdel Sattar A. Abdel Sattar

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Cairo, Dokki
Egypt

Mohamed A. Abou-Ellail

University of Aswan, Faculty of Agriculture and Natural Resources, Department of Genetics

Egypt, Aswan
Aswan, 81524
Egypt

Heba A. Mahfouze

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department

Cairo, Dokki
Egypt

Hoda M.A. Waziri

Plant Pathology Research Institute - Virus and Phytoplasma Department

Sherin A. Mahfouze (Contact Author)

National Research Centre, Genetic Engineering and Biotechnology Division, Genetics and Cytology Department ( email )

Cairo, Dokki
Egypt

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