A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a real-time machine intelligence technology based on a radically new architecture that realizes real-time image-based intelligent cell search and sorting at an unprecedented rate. The technology integrates high-throughput cell imaging, cell focusing, and cell sorting on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision making, and actuation. Specifically, we use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. Our technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.
Nitta, Nao and Sugimura, Takeaki and Isozaki, Akihiro and Mikami, Hideharu and Hiraki, Kei and Sakuma, Shinya and Iino, Takanori and Arai, Fumihito and Endo, Taichiro and Fujiwaki, Yasuhiro and Fukuzawa, Hideya and Hase, Misa and Hayakawa, Takeshi and Hiramatsu, Kotaro and Hoshino, Yu and Inaba, Mary and Ito, Takuro and Karakawa, Hiroshi and Kasai, Yusuke and Koizumi, Kenichi and Lee, SangWook and Lei, Cheng and Li, Ming and Maeno, Takanori and Matsusaka, Satoshi and Murakami, Daichi and Nakagawa, Atsuhiro and Oguchi, Yusuke and Oikawa, Minoru and Ota, Tadataka and Shiba, Kiyotaka and Shintaku, Hirofumi and Shirasaki, Yoshitaka and Suga, Kanako and Suzuki, Yuta and Suzuki, Nobutake and Tanaka, Yo and Tezuka, Hiroshi and Toyokawa, Chihana and Yalikun, Yaxiaer and Yamada, Makoto and Yamagishi, Mai and Yamano, Takashi and Yasumoto, Atsushi and Yatomi, Yutaka and Yazawa, Masayuki and Di Carlo, Dino and Hosokawa, Yoichiroh and Uemura, Sotaro and Ozeki, Yasuyuki and Goda, Keisuke, Intelligent Cell Search Engine (2018). Available at SSRN: https://ssrn.com/abstract=3204560 or http://dx.doi.org/10.2139/ssrn.3204560
This is a paper under consideration at Cell Press and has not been peer-reviewed.
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