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Intelligent Cell Search Engine

39 Pages Posted: 7 Jan 2019 Sneak Peek Status: Published

See all articles by Nao Nitta

Nao Nitta

Japan Science and Technology Agency (JST)

Takeaki Sugimura

Japan Science and Technology Agency (JST)

Akihiro Isozaki

University of Tokyo - Department of Chemistry

Hideharu Mikami

University of Tokyo - Department of Chemistry

Kei Hiraki

University of Tokyo - Department of Chemistry

Shinya Sakuma

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Takanori Iino

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Fumihito Arai

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Taichiro Endo

Riken Advanced Intelligence Project

Yasuhiro Fujiwaki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Hideya Fukuzawa

Kyoto University - Graduate School of Biostudies

Misa Hase

University of Tokyo - Department of Chemistry

Takeshi Hayakawa

Chuo University - Department of Precision Mechanics

Kotaro Hiramatsu

University of Tokyo - Department of Chemistry

Yu Hoshino

Kyushu University - Department of Chemical Engineering

Mary Inaba

University of Tokyo - Department of Creative Informatics

Takuro Ito

Japan Science and Technology Agency (JST)

Hiroshi Karakawa

University of Tokyo - Department of Chemistry

Yusuke Kasai

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Kenichi Koizumi

University of Tokyo - Department of Creative Informatics

SangWook Lee

University of Tokyo - Department of Chemistry

Cheng Lei

University of Tokyo - Department of Chemistry

Ming Li

University of California, Los Angeles (UCLA) - Department of Bioengineering

Takanori Maeno

University of Tokyo - Institute of Medical Science

Satoshi Matsusaka

Japanese Foundation for Cancer Research - Department of Gastroenterology

Daichi Murakami

University of Tokyo - Department of Creative Informatics

Atsuhiro Nakagawa

Tohoku University - Department of Neurosurgery

Yusuke Oguchi

University of Tokyo - Department of Biological Sciences

Minoru Oikawa

Kochi University - Natural Sciences Cluster

Tadataka Ota

University of Tokyo - Department of Chemistry

Kiyotaka Shiba

Japanese Foundation for Cancer Research - Division of Protein Engineering

Hirofumi Shintaku

Kyoto University - Department of Micro Engineering

Yoshitaka Shirasaki

University of Tokyo - Department of Biological Sciences

Kanako Suga

Japanese Foundation for Cancer Research - Division of Protein Engineering

Yuta Suzuki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Nobutake Suzuki

University of Tokyo - Department of Biological Sciences

Yo Tanaka

RIKEN Quantitative Biology Center

Hiroshi Tezuka

University of Tokyo - Department of Creative Informatics

Chihana Toyokawa

Kyoto University - Graduate School of Biostudies

Yaxiaer Yalikun

RIKEN Quantitative Biology Center

Makoto Yamada

Riken Advanced Intelligence Project

Mai Yamagishi

University of Tokyo - Department of Biological Sciences

Takashi Yamano

Kyoto University - Graduate School of Biostudies

Atsushi Yasumoto

University of Tokyo - Department of Clinical Laboratory Medicine

Yutaka Yatomi

University of Tokyo - Department of Clinical Laboratory Medicine

Masayuki Yazawa

Columbia University - Department of Rehabilitation and Regenerative Medicine

Dino Di Carlo

University of Tokyo - Department of Chemistry

Yoichiroh Hosokawa

Nara Institute of Science and Technology - Graduate School of Materials Science

Sotaro Uemura

University of Tokyo - Department of Biological Sciences

Yasuyuki Ozeki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Keisuke Goda

University of Tokyo - Department of Chemistry

More...

Abstract

A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a real-time machine intelligence technology based on a radically new architecture that realizes real-time image-based intelligent cell search and sorting at an unprecedented rate. The technology integrates high-throughput cell imaging, cell focusing, and cell sorting on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision making, and actuation. Specifically, we use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. Our technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

Suggested Citation

Nitta, Nao and Sugimura, Takeaki and Isozaki, Akihiro and Mikami, Hideharu and Hiraki, Kei and Sakuma, Shinya and Iino, Takanori and Arai, Fumihito and Endo, Taichiro and Fujiwaki, Yasuhiro and Fukuzawa, Hideya and Hase, Misa and Hayakawa, Takeshi and Hiramatsu, Kotaro and Hoshino, Yu and Inaba, Mary and Ito, Takuro and Karakawa, Hiroshi and Kasai, Yusuke and Koizumi, Kenichi and Lee, SangWook and Lei, Cheng and Li, Ming and Maeno, Takanori and Matsusaka, Satoshi and Murakami, Daichi and Nakagawa, Atsuhiro and Oguchi, Yusuke and Oikawa, Minoru and Ota, Tadataka and Shiba, Kiyotaka and Shintaku, Hirofumi and Shirasaki, Yoshitaka and Suga, Kanako and Suzuki, Yuta and Suzuki, Nobutake and Tanaka, Yo and Tezuka, Hiroshi and Toyokawa, Chihana and Yalikun, Yaxiaer and Yamada, Makoto and Yamagishi, Mai and Yamano, Takashi and Yasumoto, Atsushi and Yatomi, Yutaka and Yazawa, Masayuki and Di Carlo, Dino and Hosokawa, Yoichiroh and Uemura, Sotaro and Ozeki, Yasuyuki and Goda, Keisuke, Intelligent Cell Search Engine (2018). Available at SSRN: https://ssrn.com/abstract=3204560 or http://dx.doi.org/10.2139/ssrn.3204560
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Nao Nitta

Japan Science and Technology Agency (JST)

Kawaguchi Center Building
4-1-8, Honcho, Kawaguchi-shi
Saitama, 332-0012
Japan

Takeaki Sugimura

Japan Science and Technology Agency (JST)

Kawaguchi Center Building
4-1-8, Honcho, Kawaguchi-shi
Saitama, 332-0012
Japan

Akihiro Isozaki

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Hideharu Mikami

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Kei Hiraki

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Shinya Sakuma

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Furo-cho, Chikusa-ku
Nagoya-City, 4648601
Japan

Takanori Iino

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Fumihito Arai

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Furo-cho, Chikusa-ku
Nagoya-City, 4648601
Japan

Taichiro Endo

Riken Advanced Intelligence Project

Nihonbashi 1-chome Mitsui Building, 15th floor
1-4-1 Nihonbashi, Chuo-ku
Tokyo, 103-0027
Japan

Yasuhiro Fujiwaki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Hideya Fukuzawa

Kyoto University - Graduate School of Biostudies

Yoshida-Honmachi
Sakyo-ku
Kyoto, 606-8501
Japan

Misa Hase

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Takeshi Hayakawa

Chuo University - Department of Precision Mechanics

2nd floor Blg 4 Tampa Campus
742-1 Higashinakano, Hachioji
Tokyo 192-03
Japan

Kotaro Hiramatsu

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yu Hoshino

Kyushu University - Department of Chemical Engineering

6-19-1, Hakozaki, Higashiku
Fukuoka, 812-8581
Japan

Mary Inaba

University of Tokyo - Department of Creative Informatics

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Takuro Ito

Japan Science and Technology Agency (JST)

Kawaguchi Center Building
4-1-8, Honcho, Kawaguchi-shi
Saitama, 332-0012
Japan

Hiroshi Karakawa

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yusuke Kasai

Nagoya University - Department of Micro-Nano Mechanical Science and Engineering

Furo-cho, Chikusa-ku
Nagoya-City, 4648601
Japan

Kenichi Koizumi

University of Tokyo - Department of Creative Informatics

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

SangWook Lee

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Cheng Lei

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Ming Li

University of California, Los Angeles (UCLA) - Department of Bioengineering

410 Westwood Plaza
Los Angeles, CA 90095-160
United States

Takanori Maeno

University of Tokyo - Institute of Medical Science

4-6-1 Shirokanedai
Minato-ku
Tokyo, 108-8639
Japan

Satoshi Matsusaka

Japanese Foundation for Cancer Research - Department of Gastroenterology

3-8-31, Ariake, Koto
Tokyo, 135-8550
Japan

Daichi Murakami

University of Tokyo - Department of Creative Informatics

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Atsuhiro Nakagawa

Tohoku University - Department of Neurosurgery

SKK Building, Katahira 2
Aoba-ku, Sendai, Miyagi 980-8577
Japan

Yusuke Oguchi

University of Tokyo - Department of Biological Sciences

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Minoru Oikawa

Kochi University - Natural Sciences Cluster

Okocho Kohasu
Nankoku, Kochi Prefecture 783-8505
Japan

Tadataka Ota

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Kiyotaka Shiba

Japanese Foundation for Cancer Research - Division of Protein Engineering

3-8-31, Ariake, Koto
Tokyo, 135-8550
United States

Hirofumi Shintaku

Kyoto University - Department of Micro Engineering

Yoshida-Honmachi
Sakyo-ku
Kyoto, 606-8501
Japan

Yoshitaka Shirasaki

University of Tokyo - Department of Biological Sciences

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Kanako Suga

Japanese Foundation for Cancer Research - Division of Protein Engineering

3-8-31, Ariake, Koto
Tokyo, 135-8550
United States

Yuta Suzuki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Nobutake Suzuki

University of Tokyo - Department of Biological Sciences

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yo Tanaka

RIKEN Quantitative Biology Center

Osaka 565-0871
Japan

Hiroshi Tezuka

University of Tokyo - Department of Creative Informatics

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Chihana Toyokawa

Kyoto University - Graduate School of Biostudies

Yoshida-Honmachi
Sakyo-ku
Kyoto, 606-8501
Japan

Yaxiaer Yalikun

RIKEN Quantitative Biology Center

Osaka 565-0871
Japan

Makoto Yamada

Riken Advanced Intelligence Project

Nihonbashi 1-chome Mitsui Building, 15th floor
1-4-1 Nihonbashi, Chuo-ku
Tokyo, 103-0027
Japan

Mai Yamagishi

University of Tokyo - Department of Biological Sciences

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Takashi Yamano

Kyoto University - Graduate School of Biostudies

Yoshida-Honmachi
Sakyo-ku
Kyoto, 606-8501
Japan

Atsushi Yasumoto

University of Tokyo - Department of Clinical Laboratory Medicine

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yutaka Yatomi

University of Tokyo - Department of Clinical Laboratory Medicine

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Masayuki Yazawa

Columbia University - Department of Rehabilitation and Regenerative Medicine

630 West 168th St.
New York, NY 10032
United States

Dino Di Carlo

University of Tokyo - Department of Chemistry

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yoichiroh Hosokawa

Nara Institute of Science and Technology - Graduate School of Materials Science

Ikoma, 630-0192
Japan

Sotaro Uemura

University of Tokyo - Department of Biological Sciences

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Yasuyuki Ozeki

University of Tokyo - Department of Electrical Engineering and Information Systems (EEIS)

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

Keisuke Goda (Contact Author)

University of Tokyo - Department of Chemistry ( email )

Yayoi 1-1-1
Bunkyo-ku
Tokyo, 113-8657
Japan

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