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Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression

44 Pages Posted: 18 Jan 2019 Sneak Peek Status: Published

See all articles by Rajendra K. Gangalum

Rajendra K. Gangalum

University of California, Los Angeles (UCLA) - Stein Eye Institute

Dongjae Kim

University of California, Los Angeles (UCLA) - Stein Eye Institute

Raj K. Kashyap

University of California, Los Angeles (UCLA) - Stein Eye Institute

Serghei Mangul

University of California, Los Angeles (UCLA) - Department of Computer Science and Human Genetics

Xinkai Zhou

University of California, Los Angeles (UCLA) - Department of Medicine

David Elashoff

University of California, Los Angeles (UCLA) - Department of Medicine

Suraj Bhat

University of California, Los Angeles (UCLA) - Stein Eye Institute; University of California, Los Angeles (UCLA) - Brain Research Institute; University of California, Los Angeles (UCLA) - Molecular Biology Institute

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Abstract

The developing eye lens presents an exceptional paradigm for spatial transcriptomics. It is composed of highly organized long, slender transparent fiber cells, which differentiate from the edges of the anterior epithelium of the lens (equator), attended by high expression of crystallins, which generate transparency. Every fiber cell, therefore, is an optical unit whose refractive properties derive from its gene activity. Here, we probe this tangible relationship between the gene activity and the phenotype by studying the expression of all known 17 crystallins and 77 other non-crystallin genes in single fiber cells isolated from three states/regions of differentiation allowing us to follow the progression of differentiation at the single cell level. The data demonstrates highly variable gene activity, in cortical fibers, interposed between the nascent and the terminally differentiated fiber-cell transcription. This data suggests that the so-called “Stochastic”, highly heterogeneous gene activity is a regulated intermediate in the progression towards the realization of a functional phenotype.

Suggested Citation

Gangalum, Rajendra K. and Kim, Dongjae and Kashyap, Raj K. and Mangul, Serghei and Zhou, Xinkai and Elashoff, David and Bhat, Suraj, Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression (2018). Available at SSRN: https://ssrn.com/abstract=3205412 or http://dx.doi.org/10.2139/ssrn.3205412
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Rajendra K. Gangalum

University of California, Los Angeles (UCLA) - Stein Eye Institute

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

Dongjae Kim

University of California, Los Angeles (UCLA) - Stein Eye Institute

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

Raj K. Kashyap

University of California, Los Angeles (UCLA) - Stein Eye Institute

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

Serghei Mangul

University of California, Los Angeles (UCLA) - Department of Computer Science and Human Genetics

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

Xinkai Zhou

University of California, Los Angeles (UCLA) - Department of Medicine

Los Angeles, CA
United States

David Elashoff

University of California, Los Angeles (UCLA) - Department of Medicine

Los Angeles, CA
United States

Suraj Bhat (Contact Author)

University of California, Los Angeles (UCLA) - Stein Eye Institute ( email )

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

University of California, Los Angeles (UCLA) - Brain Research Institute

Box 951761
Los Angeles, CA 90095-1761
United States

University of California, Los Angeles (UCLA) - Molecular Biology Institute

405 Hilgard Avenue
Box 951361
Los Angeles, CA 90095
United States

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