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Lactobacillus Casei Protects Dextran Sodium Sulfate- or Rapamycin-Induced Colonic Inflammation in the Mouse

29 Pages Posted: 10 Sep 2018

See all articles by Yong Zhang

Yong Zhang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Qiangchuan Hou

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Chen Ma

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Jie Zhao

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Haiyan Xu

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Weicheng Li

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Yanjie Wang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Huimin Ma

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Heping Zhang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

Zhihong Sun

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

More...

Abstract

Human colon inflammation is associated with changes in the diverse and abundant microorganisms in the gut. As important beneficial microbes, Lactobacillus contributes to the immune responses and intestinal integrity that may alleviate experimental colitis. However, the mechanisms underlying probiotic benefits have not been fully elucidated. The objective of this study was to assess the impact of Lactobacillus casei Zhang (LCZ) intake on immune and chloride ion channel profiles and to examine its association with gut microbial changes in dextran sodium sulfate or rapamycin-challenged mice. Histological evaluation of the colon, levels of colonic myeloperoxidase, serum tumor necrosis factor-α and interleukin-6 were assessed as inflammatory markers and the gut microbiota profiles of each mouse were studied. We found that LCZ can prevent experimental colitis and rapamycin-induced inflammation in intestinal mucosa by improving histological scores, decreasing host inflammatory cytokines, modulating gut-dominated bacteria, enhancing cystic fibrosis transmembrane conductance regulator expression and blocking interleukin-6 /p-STAT3 or Akt/NF-κB signaling. Our results suggest that LCZ may provide effective prevention against colitis.

Funding Statement: Financial support for this research was provided by the National Natural Science Foundation of China (31720103911 and 31622043).

Declaration of Interests: The authors declare no conflict of interest.

Ethics Approval Statement: All animal protocols were approved by the Institutional Animal Care and Use Committee of the Third Military Medical University.

Keywords: LCZ, Colitis, Prevention, CFTR

Suggested Citation

Zhang, Yong and Hou, Qiangchuan and Ma, Chen and Zhao, Jie and Xu, Haiyan and Li, Weicheng and Wang, Yanjie and Ma, Huimin and Zhang, Heping and Sun, Zhihong, Lactobacillus Casei Protects Dextran Sodium Sulfate- or Rapamycin-Induced Colonic Inflammation in the Mouse (December 7, 2018). Available at SSRN: https://ssrn.com/abstract=3213664

Yong Zhang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Qiangchuan Hou

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Chen Ma

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Jie Zhao

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Haiyan Xu

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Weicheng Li

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Yanjie Wang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Huimin Ma

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Heping Zhang

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

Zhihong Sun (Contact Author)

Inner Mongolia Agricultural University - Key Laboratory of Dairy Biotechnology and Engineering ( email )

306 Zhaowuda Rd, Saihan Qu
Huhehaote Shi
Neimenggu Zizhiqu, 01000
China

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