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An Organ-Wide Gene Expression Atlas of the Developing Human Heart

79 Pages Posted: 24 Jul 2018 Sneak Peek Status: Review Complete

See all articles by Michaela Asp

Michaela Asp

Royal Institute of Technology (KTH) - Department of Gene Technology

Stefania Giacomello

Stockholm University - Department of Biochemistry and Biophysics

Daniel Fürth

Cold Spring Harbor Laboratory

Johan Reimegård

Uppsala University - Department of Cell and Molecular Biology

Eva Wärdell

Karolinska Institutet - Department of Medicine

Joaquin Custodio

Karolinska Institutet - Department of Medical Biochemistry and Biophysics

Fredrik Salmén

Royal Netherlands Academy of Arts and Sciences - Hubrecht Institute‐KNAW

Erik Sundström

Karolinska Institutet - Department of Neurobiology

Elisabet Åkesson

Karolinska Institutet - Department of Neurobiology

Magda Bienko

Karolinska Institutet - Department of Medical Biochemistry and Biophysics

Agneta Månsson‐Broberg

Karolinska Institutet - Department of Medicine

Patrik L. Ståhl

Royal Institute of Technology (KTH) - Department of Gene Technology

Christer Sylvén

Uppsala University - Department of Cell and Molecular Biology

Joakim Lundeberg

Royal Institute of Technology (KTH) - Department of Gene Technology

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Abstract

The human developing heart holds a greater proportion of stem-cell-like cells than the adult heart. However, it is not completely understood how these stem cells differentiate into various cardiac cell types. We have performed an organ-wide transcriptional landscape analysis of the developing heart to advance our understanding of cardiac morphogenesis in humans. Comprehensive spatial gene expression analyses identified distinct profiles that correspond not only to individual chamber compartments, but also distinctive regions within the outflow tract. Furthermore, the generated spatial expression reference maps facilitated the assignment of 3,787 human embryonic cardiac cells obtained from single-cell RNA-sequencing to an in situ location. Through this approach we reveal that the outflow tract contains a wider range of cell types than the chambers, and that the epicardium expression profile can be traced to several cell types that are activated at different stages of development. We also provide a 3D spatial model of human embryonic cardiac cells to enable further studies of the developing human heart.

Suggested Citation

Asp, Michaela and Giacomello, Stefania and Fürth, Daniel and Reimegård, Johan and Wärdell, Eva and Custodio, Joaquin and Salmén, Fredrik and Sundström, Erik and Åkesson, Elisabet and Bienko, Magda and Månsson‐Broberg, Agneta and Ståhl, Patrik L. and Sylvén, Christer and Lundeberg, Joakim, An Organ-Wide Gene Expression Atlas of the Developing Human Heart (2018). Available at SSRN: https://ssrn.com/abstract=3219263 or http://dx.doi.org/10.2139/ssrn.3219263
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Michaela Asp

Royal Institute of Technology (KTH) - Department of Gene Technology

Lindstedtsvägen 30-100 44
Stockholm, SE-100 44
Sweden

Stefania Giacomello

Stockholm University - Department of Biochemistry and Biophysics

Universitetsvägen 10
Stockholm, Stockholm SE-106 91
Sweden

Daniel Fürth

Cold Spring Harbor Laboratory

Cold Spring Harbor, NY 11724
United States

Johan Reimegård

Uppsala University - Department of Cell and Molecular Biology

Box 513
Uppsala, 751 20
Sweden

Eva Wärdell

Karolinska Institutet - Department of Medicine

Stockholm, SE-171 76
Sweden

Joaquin Custodio

Karolinska Institutet - Department of Medical Biochemistry and Biophysics

Granits väg 4
SE-171 77 Stockholm, Stockholm 17171
Sweden

Fredrik Salmén

Royal Netherlands Academy of Arts and Sciences - Hubrecht Institute‐KNAW

Amsterdam, 1000 GC
Netherlands

Erik Sundström

Karolinska Institutet - Department of Neurobiology

Granits väg 4
SE-171 77 Stockholm, Stockholm 17171
Sweden

Elisabet Åkesson

Karolinska Institutet - Department of Neurobiology

Granits väg 4
SE-171 77 Stockholm, Stockholm 17171
Sweden

Magda Bienko

Karolinska Institutet - Department of Medical Biochemistry and Biophysics

Granits väg 4
SE-171 77 Stockholm, Stockholm 17171
Sweden

Agneta Månsson‐Broberg

Karolinska Institutet - Department of Medicine

Stockholm, SE-171 76
Sweden

Patrik L. Ståhl

Royal Institute of Technology (KTH) - Department of Gene Technology

Lindstedtsvägen 30-100 44
Stockholm, SE-100 44
Sweden

Christer Sylvén

Uppsala University - Department of Cell and Molecular Biology

Box 513
Uppsala, 751 20
Sweden

Joakim Lundeberg (Contact Author)

Royal Institute of Technology (KTH) - Department of Gene Technology ( email )

Lindstedtsvägen 30-100 44
Stockholm, SE-100 44
Sweden

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