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Rapid and Sensitive Genotyping of Multiple Clinical Samples Based on a Combined Direct PCR and Magnetic Lateral Flow Assay

40 Pages Posted: 26 Jul 2018 Publication Status: Published

See all articles by Chao Zhang

Chao Zhang

Northwest University (China) - College of Life Sciences

Xiaonan Liu

Northwest University (China) - College of Life Sciences

Yao Yao

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Kewu Liu

Northwest University (China) - College of Life Sciences

Wenli Hui

Northwest University (China) - College of Life Sciences

Juanli Zhu

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Yaling Dou

Chinese Academy of Medical Sciences - Department of Clinical Laboratory

Kai Hua

Northwest University (China) - College of Life Sciences

Mingli Peng

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Zuankai Wang

City University of Hong Kong (CityUHK) - Department of Mechanical Engineering

Alphonsus J. M. Vermorken

Northwest University (China) - College of Life Sciences

Yali Cui

Northwest University (China) - College of Life Sciences

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Abstract

Developing a sensitive, low cost and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0×102 cells/μL, which are better than those of the gold standard methods. We believe the direct PCR-MLFA system represents a significant advance towards the development of portable, sensitive biomedical platforms.

Suggested Citation

Zhang, Chao and Liu, Xiaonan and Yao, Yao and Liu, Kewu and Hui, Wenli and Zhu, Juanli and Dou, Yaling and Hua, Kai and Peng, Mingli and Wang, Zuankai and Vermorken, Alphonsus J. M. and Cui, Yali, Rapid and Sensitive Genotyping of Multiple Clinical Samples Based on a Combined Direct PCR and Magnetic Lateral Flow Assay (2018). Available at SSRN: https://ssrn.com/abstract=3219289 or http://dx.doi.org/10.2139/ssrn.3219289
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Chao Zhang

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Xiaonan Liu

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Yao Yao

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Xi’an
China

Kewu Liu

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Wenli Hui

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Juanli Zhu

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Xi’an
China

Yaling Dou

Chinese Academy of Medical Sciences - Department of Clinical Laboratory

NO. 9, Dongdan Santiao
Beijing, Dongcheng District
China

Kai Hua

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Mingli Peng

Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection

Xi’an
China

Zuankai Wang (Contact Author)

City University of Hong Kong (CityUHK) - Department of Mechanical Engineering ( email )

83 Tat Chee Avenue
Kowloon
Hong Kong

Alphonsus J. M. Vermorken

Northwest University (China) - College of Life Sciences

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

Yali Cui

Northwest University (China) - College of Life Sciences ( email )

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

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