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MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

79 Pages Posted: 2 Aug 2018 Sneak Peek Status: Review Complete

See all articles by Mayeul Collot

Mayeul Collot

University of Strasbourg - Biophotonics Laboratory and Pathology

Pichandi Ashokkumar

University of Strasbourg - Biophotonics Laboratory and Pathology

Halina Anton

University of Strasbourg - Biophotonics Laboratory and Pathology

Emmanuel  Boutant

University of Strasbourg - Biophotonics Laboratory and Pathology

Orestis Faklaris

Université Paris VII Denis Diderot - Institut Jacques Monod

Thierry Galli

Université Paris VII Denis Diderot - Membrane Traffic in Health and Disease

Yves Mély

University of Strasbourg - Biophotonics Laboratory and Pathology

Lydia Danglot

Université Paris VII Denis Diderot - Institut Jacques Monod

Andrey S. Klymchenko

University of Strasbourg - Biophotonics Laboratory and Pathology

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Abstract

The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the cell. Herein, we developed MemBright: a family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near-infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono and two-photon microscopy. These probes are compatible with long-term live cell imaging and immunostaining. Moreover, MemBright label neurons in a brighter manner than surrounding cells allowing identification of neurons in acute brain tissue section and neuromuscular-junctions without any use of transfection or transgenic animals. At last, MemBright were used in super-resolution imaging to unravel the dendritic spines’ neck. 3D multicolor dSTORM in combination with immunostaining revealed en-passant synapse displaying endogenous glutamate receptors clustered at the axonal-dendritic contact site. MemBright probes thus constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques.

Suggested Citation

Collot, Mayeul and Ashokkumar, Pichandi and Anton, Halina and Boutant, Emmanuel  and Faklaris, Orestis and Galli, Thierry and Mély, Yves and Danglot, Lydia and Klymchenko, Andrey S., MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience (2018). Available at SSRN: https://ssrn.com/abstract=3224530 or http://dx.doi.org/10.2139/ssrn.3224530
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Mayeul Collot (Contact Author)

University of Strasbourg - Biophotonics Laboratory and Pathology ( email )

Strasbourg, Alsace
France

Pichandi Ashokkumar

University of Strasbourg - Biophotonics Laboratory and Pathology

Strasbourg, Alsace
France

Halina Anton

University of Strasbourg - Biophotonics Laboratory and Pathology

Strasbourg, Alsace
France

Emmanuel  Boutant

University of Strasbourg - Biophotonics Laboratory and Pathology

Strasbourg, Alsace
France

Orestis Faklaris

Université Paris VII Denis Diderot - Institut Jacques Monod

2, place Jussieu
Paris, 75005
France

Thierry Galli

Université Paris VII Denis Diderot - Membrane Traffic in Health and Disease ( email )

2, place Jussieu
Paris, 75005
France

Yves Mély

University of Strasbourg - Biophotonics Laboratory and Pathology

Strasbourg, Alsace
France

Lydia Danglot

Université Paris VII Denis Diderot - Institut Jacques Monod

2, place Jussieu
Paris, 75005
France

Andrey S. Klymchenko

University of Strasbourg - Biophotonics Laboratory and Pathology

Strasbourg, Alsace
France