lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Integration of QsvR into the Quorum Sensing Circuit of Vibrio parahaemolyticus

42 Pages Posted: 4 Oct 2018

See all articles by Yiquan Zhang

Yiquan Zhang

Jiangsu University

Lingfei Hu

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Yue Qiu

Jiangsu University

George Osei-Adjei

Jiangsu University

Hao Tang

Jiangsu University

Ying Zhang

Jiangsu University

Rui Zhang

Jiangsu University

Xiumei Sheng

Jiangsu University

Shungao Xu

Jiangsu University

Wehui Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Huiying Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Zhe Yin

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Ruifu Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Xinxiang Huang

Jiangsu University

Dongsheng Zhou

Academy of Military Sciences - State Key Laboratory of Pathogen and Biosecurity

More...

Abstract

Vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis worldwide, requires the two type-III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) to infect the small intestine. The tdh genes and the T3SS2 gene cluster constitute an 80-kb pathogenicity island known as Vp-PAI located on the chromosome II. Expression of T3SS1 and Vp-PAI is regulated in a quorum sensing (QS)-dependent manner but lacks the detailed regulatory mechanisms. Herein, we show that three factors (QS regulators AphA and OpaR and novel regulator QsvR) form a complex regulatory network to regulate the T3SS1 and Vp-PAI genes. At a low cell density, the expression of the T3SS1 genes is induced by the direct binding of AphA to the exsBAD-vscBCD operon, while Vp-PAI expression is repressed. At a high cell density (HCD), the bacterium turns off T3SS1 expression by replacing AphA with OpaR, triggering the induction of Vp-PAI. Furthermore, QsvR binds to the regulatory regions of all the tested Vp-PAI genes to activate their transcription at HCD. Taken together, our data highlight how multiple QS regulators contribute to the pathogenicity of V. parahaemolyticus by precisely controlling the expression of major virulence determinants during different stages of infection.

Funding Statement: This work was supported by the National Natural Science Foundation of China (grant numbers 31671290 and 81601809)

Declaration of Interests: The authors declared that they have no conflicts of interest to this work.

Ethics Approval Statement: All the animal experiments were approved by the Committee on Animal Research of the Academy of Military Medical Sciences and carried out per the approved guideline.

Keywords: Vibrio parahaemolyticus; Quorum Sensing; AphA; OpaR; QsvR; T3SS1; Vp-PAI

Suggested Citation

Zhang, Yiquan and Hu, Lingfei and Qiu, Yue and Osei-Adjei, George and Tang, Hao and Zhang, Ying and Zhang, Rui and Sheng, Xiumei and Xu, Shungao and Yang, Wehui and Yang, Huiying and Yin, Zhe and Yang, Ruifu and Huang, Xinxiang and Zhou, Dongsheng, Integration of QsvR into the Quorum Sensing Circuit of Vibrio parahaemolyticus (September 17, 2018). Available at SSRN: https://ssrn.com/abstract=3250922 or http://dx.doi.org/10.2139/ssrn.3250922

Yiquan Zhang

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Lingfei Hu

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Beijing, 100071
China

Yue Qiu

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

George Osei-Adjei

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Hao Tang

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Ying Zhang

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Rui Zhang

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Xiumei Sheng

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Shungao Xu

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Wehui Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Beijing, 100071
China

Huiying Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology ( email )

Beijing, 100071
China

Zhe Yin

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Beijing, 100071
China

Ruifu Yang

Academy of Military Sciences - Beijing Institute of Microbiology and Epidemiology

Beijing, 100071
China

Xinxiang Huang

Jiangsu University

Xuefu Rd. 301
Xhenjiang, CA Jiangsu Province 212013
China

Dongsheng Zhou (Contact Author)

Academy of Military Sciences - State Key Laboratory of Pathogen and Biosecurity ( email )

Beijing, 100071
China

Click here to go to TheLancet.com

Paper statistics

Abstract Views
138
Downloads
11